48453 20200221144118.0 doi 10.1371/journal.pone.0153028 sideral 94406 ART-2016-94406 eng (orcid)0000-0001-7866-2803 Uranga, S Universidad de Zaragoza Granzyme A Is Expressed in Mouse Lungs during Mycobacterium tuberculosis Infection but Does Not Contribute to Protection In Vivo 2016 Access copy available to the general public Unrestricted Granzyme A, a serine protease expressed in the granules of cytotoxic T and Natural Killer cells, is involved in the generation of pro-inflammatory cytokines by macrophages. Granzyme A has been described to induce in macrophages in vitro the activation of pro-inflammatory pathways that impair intracellular mycobacterial replication. In the present study, we explored the physiological relevance of Granzyme A in the control of pulmonary Mycobacterium tuberculosis infection in vivo. Our results show that, even though Granzyme A is expressed by cytotoxic cells from mouse lungs during pulmonary infection, its deficiency in knockout mice does not have an effect in the control of M. tuberculosis infection. In addition our findings indicate that absence of Granzyme A does not affect the protection conferred by the live-attenuated M. tuberculosis vaccine MTBVAC. Altogether, our findings are in apparent contradiction with previously published in vitro results and suggest that Granzyme A does not have a crucial role in vivo in the protective response to tuberculosis. info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/BIO2014-5258P This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 643381-TBVAC2020 info:eu-repo/grantAgreement/EC/H2020/643381/EU/TBVAC2020; Advancing novel and promising TB vaccine candidates from discovery to preclinical and early clinical development/TBVAC2020 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.806 2016 MULTIDISCIPLINARY SCIENCES 15 / 63 = 0.238 2016 Q1 T1 1.236 2016 Agricultural and Biological Sciences (miscellaneous) 2016 Q1 Medicine (miscellaneous) 2016 Q1 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0001-8644-120X Marinova, D Universidad de Zaragoza (orcid)0000-0003-2993-5478 Martin, C Universidad de Zaragoza (orcid)0000-0003-0154-0730 Pardo, J Universidad de Zaragoza (orcid)0000-0001-7897-9173 Aguilo, N Universidad de Zaragoza 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 1008 X Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Proy. investigación HQA 11, 4 (2016), [8 pp] PLoS One PloS one 1932-6203 529628 http://zaguan.unizar.es/record/48453/files/texto_completo.pdf Versión publicada 104644 http://zaguan.unizar.es/record/48453/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:48453 articulos driver 2020-02-21-13:02:26 ARTICLE