000048454 001__ 48454
000048454 005__ 20200221144323.0
000048454 0247_ $$2doi$$a10.1016/j.cmpb.2016.03.019
000048454 0248_ $$2sideral$$a93839
000048454 037__ $$aART-2016-93839
000048454 041__ $$aeng
000048454 100__ $$0(orcid)0000-0003-0509-1450$$aMousavi, Seyed Jamaleddin
000048454 245__ $$aNumerical modeling of cell differentiation and proliferation in force-induced substrates via encapsulated magnetic nanoparticles
000048454 260__ $$c2016
000048454 5060_ $$aAccess copy available to the general public$$fUnrestricted
000048454 5203_ $$aBackground and objective
Cell migration, differentiation, proliferation and apoptosis are the main processes in tissue regeneration. Mesenchymal Stem Cells have the potential to differentiate into many cell phenotypes such as tissue- or organ-specific cells to perform special functions. Experimental observations illustrate that differentiation and proliferation of these cells can be regulated according to internal forces induced within their Extracellular Matrix. The process of how exactly they interpret and transduce these signals is not well understood.
Methods
A previously developed three-dimensional (3D) computational model is here extended and employed to study how force-free substrates and force-induced substrate control cell differentiation and/or proliferation during the mechanosensing process. Consistent with experimental observations, it is assumed that cell internal deformation (a mechanical signal) in correlation with the cell maturation state directly triggers cell differentiation and/or proliferation. The Extracellular Matrix is modeled as Neo-Hookean hyperelastic material assuming that cells are cultured within 3D nonlinear hydrogels.
Results
In agreement with well-known experimental observations, the findings here indicate that within neurogenic (0.1–1 kPa), chondrogenic (20–25 kPa) and osteogenic (30–45 kPa) substrates, Mesenchymal Stem Cells differentiation and proliferation can be precipitated by inducing the substrate with an internal force. Therefore, cells require a longer time to grow and maturate within force-free substrates than within force-induced substrates. In the instance of Mesenchymal Stem Cells differentiation into a compatible phenotype, the magnitude of the net traction force increases within chondrogenic and osteogenic substrates while it reduces within neurogenic substrates. This is consistent with experimental studies and numerical works recently published by the same authors. However, in all cases the magnitude of the net traction force considerably increases at the instant of cell proliferation because of cell–cell interaction.
Conclusions
The present model provides new perspectives to delineate the role of force-induced substrates in remotely controlling the cell fate during cell–matrix interaction, which open the door for new tissue regeneration methodologies.
000048454 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/MAT2013-46467-C4-3-R
000048454 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000048454 590__ $$a2.503$$b2016
000048454 591__ $$aCOMPUTER SCIENCE, THEORY & METHODS$$b21 / 104 = 0.202$$c2016$$dQ1$$eT1
000048454 591__ $$aMEDICAL INFORMATICS$$b8 / 23 = 0.348$$c2016$$dQ2$$eT2
000048454 591__ $$aENGINEERING, BIOMEDICAL$$b26 / 77 = 0.338$$c2016$$dQ2$$eT2
000048454 591__ $$aCOMPUTER SCIENCE, INTERDISCIPLINARY APPLICATIONS$$b32 / 105 = 0.305$$c2016$$dQ2$$eT1
000048454 592__ $$a0.639$$b2016
000048454 593__ $$aComputer Science Applications$$c2016$$dQ2
000048454 593__ $$aSoftware$$c2016$$dQ2
000048454 593__ $$aHealth Informatics$$c2016$$dQ2
000048454 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000048454 700__ $$0(orcid)0000-0003-0088-7222$$aHamdy Doweidar, Mohamed$$uUniversidad de Zaragoza
000048454 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000048454 773__ $$g130 (2016), 106-117$$pComput. methods programs biomed.$$tComputer Methods and Programs in Biomedicine$$x0169-2607
000048454 8564_ $$s596226$$uhttps://zaguan.unizar.es/record/48454/files/texto_completo.pdf$$yPostprint
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000048454 951__ $$a2020-02-21-13:42:42
000048454 980__ $$aARTICLE