48454 20200221144323.0 doi 10.1016/j.cmpb.2016.03.019 sideral 93839 ART-2016-93839 eng (orcid)0000-0003-0509-1450 Mousavi, Seyed Jamaleddin Numerical modeling of cell differentiation and proliferation in force-induced substrates via encapsulated magnetic nanoparticles 2016 Access copy available to the general public Unrestricted Background and objective Cell migration, differentiation, proliferation and apoptosis are the main processes in tissue regeneration. Mesenchymal Stem Cells have the potential to differentiate into many cell phenotypes such as tissue- or organ-specific cells to perform special functions. Experimental observations illustrate that differentiation and proliferation of these cells can be regulated according to internal forces induced within their Extracellular Matrix. The process of how exactly they interpret and transduce these signals is not well understood. Methods A previously developed three-dimensional (3D) computational model is here extended and employed to study how force-free substrates and force-induced substrate control cell differentiation and/or proliferation during the mechanosensing process. Consistent with experimental observations, it is assumed that cell internal deformation (a mechanical signal) in correlation with the cell maturation state directly triggers cell differentiation and/or proliferation. The Extracellular Matrix is modeled as Neo-Hookean hyperelastic material assuming that cells are cultured within 3D nonlinear hydrogels. Results In agreement with well-known experimental observations, the findings here indicate that within neurogenic (0.1–1 kPa), chondrogenic (20–25 kPa) and osteogenic (30–45 kPa) substrates, Mesenchymal Stem Cells differentiation and proliferation can be precipitated by inducing the substrate with an internal force. Therefore, cells require a longer time to grow and maturate within force-free substrates than within force-induced substrates. In the instance of Mesenchymal Stem Cells differentiation into a compatible phenotype, the magnitude of the net traction force increases within chondrogenic and osteogenic substrates while it reduces within neurogenic substrates. This is consistent with experimental studies and numerical works recently published by the same authors. However, in all cases the magnitude of the net traction force considerably increases at the instant of cell proliferation because of cell–cell interaction. Conclusions The present model provides new perspectives to delineate the role of force-induced substrates in remotely controlling the cell fate during cell–matrix interaction, which open the door for new tissue regeneration methodologies. info:eu-repo/grantAgreement/ES/MINECO/MAT2013-46467-C4-3-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.503 2016 COMPUTER SCIENCE, THEORY & METHODS 21 / 104 = 0.202 2016 Q1 T1 MEDICAL INFORMATICS 8 / 23 = 0.348 2016 Q2 T2 ENGINEERING, BIOMEDICAL 26 / 77 = 0.338 2016 Q2 T2 COMPUTER SCIENCE, INTERDISCIPLINARY APPLICATIONS 32 / 105 = 0.305 2016 Q2 T1 0.639 2016 Computer Science Applications 2016 Q2 Software 2016 Q2 Health Informatics 2016 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0003-0088-7222 Hamdy Doweidar, Mohamed Universidad de Zaragoza 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 130 (2016), 106-117 Comput. methods programs biomed. Computer Methods and Programs in Biomedicine 0169-2607 596226 http://zaguan.unizar.es/record/48454/files/texto_completo.pdf Postprint 8683 http://zaguan.unizar.es/record/48454/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:48454 articulos driver 2020-02-21-13:42:42 ARTICLE