engLorenzo-Tejedor, Mónicade la Cámara Izquierdo, ConcepciónLópez Antón, RaulBailón Luesma, RaquelAguiló Llobet, JordiBernal Ruiz, María LuisaDirect infussion Electrospray Mass Spectrometry as a new non-invasive tool for serum metabolomics in induced-stress subjectsART-2015-94556Background and Objectives: Nanotechnology is becoming a tool for the study of changes in the metabolome of patients in different states of disease. Analytical techniques such as Electrospray Mass Spectrometry, allow to find biomarkers by determi- nation of metabolites. Nowadays, there is not an objective analytical approach for diagno- sis of stress. Thus, the objectives of this pilot work are: – Describing the development of a fast, direct and non-invasive analytical protocol, ap- plied for the first time, to study the metabolomic profile of patient s different states through a disease. – Testing the protocol in a pilot sample with non-stressed and stress-induced subjects. Methods: High resolution direct infusion electrospray mass spectrometry has been used to analyse the metabolome of blood samples (0.3 ml) from six subject s. Results: Data prove a clear discrimination between non-stressed and stressed states in the metabolome. Data showed different predominant metabolites in both states. Results allow objective characterization of the state of the patient. Conclusions: Although this is a pilot study, the method was successful in discriminating different metabolites in non-stressed and stress-induced subjects.2015http://zaguan.unizar.es/record/48626http://zaguan.unizar.es/record/48626oai:zaguan.unizar.es:48626EUROPEAN JOURNAL OF PSYCHIATRY 29, 4 (2015), 259-275byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess