48676 20200221144248.0 doi 10.1371/journal.pone.0151384 sideral 94643 ART-2016-94643 eng (orcid)0000-0002-0531-0943 González, A. Universidad de Zaragoza Expanding the role of FurA as essential global regulator in cyanobacteria 2016 Access copy available to the general public Unrestricted In the nitrogen-fixing heterocyst-forming cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA plays a global regulatory role. Failures to eliminate wild-type copies of furA gene from the polyploid genome suggest essential functions. In the present study, we developed a selectively regulated furA expression system by the replacement of furA promoter in the Anabaena sp. chromosomes with the Co2+/Zn2+ inducible coaT promoter from Synechocystis sp. PCC 6803. By removing Co2+ and Zn2+ from the medium and shutting off furA expression, we showed that FurA was absolutely required for cyanobacterial growth. RNA-seq based comparative transcriptome analyses of the furA-turning off strain and its parental wild-type in conjunction with subsequent electrophoretic mobility shift assays and semi-quantitative RT-PCR were carried out in order to identify direct transcriptional targets and unravel new biological roles of FurA. The results of such approaches led us to identify 15 novel direct iron-dependent transcriptional targets belonging to different functional categories including detoxification and defences against oxidative stress, phycobilisome degradation, chlorophyll catabolism and programmed cell death, light sensing and response, heterocyst differentiation, exopolysaccharide biosynthesis, among others. Our analyses evidence novel interactions in the complex regulatory network orchestrated by FurA in cyanobacteria. info:eu-repo/grantAgreement/ES/DGA/B18 info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.806 2016 MULTIDISCIPLINARY SCIENCES 15 / 63 = 0.238 2016 Q1 T1 1.236 2016 Agricultural and Biological Sciences (miscellaneous) 2016 Q1 Medicine (miscellaneous) 2016 Q1 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0002-8181-2689 Bes , M.T. Universidad de Zaragoza (orcid)0000-0002-2742-3711 Peleato, M.L. Universidad de Zaragoza (orcid)0000-0001-8644-4574 Fillat, M.F. Universidad de Zaragoza 1002 412 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Fisiología Vegetal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 11, 3 (2016), 0151384 [22 p.] PLoS One PloS one 1932-6203 1790862 http://zaguan.unizar.es/record/48676/files/texto_completo.pdf Versión publicada 103539 http://zaguan.unizar.es/record/48676/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:48676 articulos driver 2020-02-21-13:26:27 ARTICLE