000048677 001__ 48677 000048677 005__ 20200221144247.0 000048677 0247_ $$2doi$$a10.1016/j.celrep.2016.03.009 000048677 0248_ $$2sideral$$a94648 000048677 037__ $$aART-2016-94648 000048677 041__ $$aeng 000048677 100__ $$aGuarás, A. 000048677 245__ $$aThe CoQH2/CoQ Ratio Serves as a Sensor of Respiratory Chain Efficiency 000048677 260__ $$c2016 000048677 5060_ $$aAccess copy available to the general public$$fUnrestricted 000048677 5203_ $$aElectrons feed into the mitochondrial electron transport chain (mETC) from NAD- or FAD-dependent enzymes. A shift from glucose to fatty acids increases electron flux through FAD, which can saturate the oxidation capacity of the dedicated coenzyme Q (CoQ) pool and result in the generation of reactive oxygen species. To prevent this, the mETC superstructure can be reconfigured through the degradation of respiratory complex I, liberating associated complex III to increase electron flux via FAD at the expense of NAD. Here, we demonstrate that this adaptation is driven by the ratio of reduced to oxidized CoQ. Saturation of CoQ oxidation capacity induces reverse electron transport from reduced CoQ to complex I, and the resulting local generation of superoxide oxidizes specific complex I proteins, triggering their degradation and the disintegration of the complex. Thus, CoQ redox status acts as a metabolic sensor that fine-tunes mETC configuration in order to match the prevailing substrate profile. 000048677 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/SAF2012-32776$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2012-37926$$9info:eu-repo/grantAgreement/ES/MICINN/CSD2007-00020$$9info:eu-repo/grantAgreement/ES/ISCIII/RD12-0042-0056$$9info:eu-repo/grantAgreement/ES/ISCIII/PI12-01297 000048677 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000048677 590__ $$a8.282$$b2016 000048677 591__ $$aCELL BIOLOGY$$b26 / 189 = 0.138$$c2016$$dQ1$$eT1 000048677 592__ $$a8.336$$b2016 000048677 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1 000048677 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000048677 700__ $$aPerales-Clemente, E. 000048677 700__ $$aCalvo, E. 000048677 700__ $$aAcín-Pérez, R. 000048677 700__ $$aLoureiro-Lopez, M. 000048677 700__ $$aPujol, C. 000048677 700__ $$aMartínez-Carrascoso, I. 000048677 700__ $$aNuñez, E. 000048677 700__ $$aGarcía-Marqués, F. 000048677 700__ $$aRodríguez-Hernández, M.A. 000048677 700__ $$aCortés, A. 000048677 700__ $$aDiaz, F. 000048677 700__ $$0(orcid)0000-0002-8705-2799$$aPérez-Martos, A. 000048677 700__ $$aMoraes, C.T. 000048677 700__ $$0(orcid)0000-0001-8971-7355$$aFernández-Silva, P.$$uUniversidad de Zaragoza 000048677 700__ $$aTrifunovic, A. 000048677 700__ $$aNavas, P. 000048677 700__ $$aVazquez, J. 000048677 700__ $$0(orcid)0000-0002-4931-6730$$aEnríquez, J.A.$$uUniversidad de Zaragoza 000048677 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000048677 773__ $$g15, 1 (2016), 197-209$$pCell Reports$$tCell Reports$$x2211-1247 000048677 8564_ $$s3940716$$uhttps://zaguan.unizar.es/record/48677/files/texto_completo.pdf$$yVersión publicada 000048677 8564_ $$s58383$$uhttps://zaguan.unizar.es/record/48677/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000048677 909CO $$ooai:zaguan.unizar.es:48677$$particulos$$pdriver 000048677 951__ $$a2020-02-21-13:25:57 000048677 980__ $$aARTICLE