000048693 001__ 48693
000048693 005__ 20200221144200.0
000048693 0247_ $$2doi$$a10.1371/journal.pone.0155336
000048693 0248_ $$2sideral$$a94807
000048693 037__ $$aART-2016-94807
000048693 041__ $$aeng
000048693 100__ $$0(orcid)0000-0002-0308-1390$$aRamo, A.$$uUniversidad de Zaragoza
000048693 245__ $$aIntra-species genetic diversity and clonal structure of Cryptosporidium parvum in sheep farms in a confined geographical area in northeastern Spain
000048693 260__ $$c2016
000048693 5060_ $$aAccess copy available to the general public$$fUnrestricted
000048693 5203_ $$aA multilocus fragment typing approach including eleven variable-number tandem-repeat (VNTR) loci and the GP60 gene was used to investigate the intra-farm and intra-host genetic diversity of Cryptosporidium parvum in sheep farms in a confined area in northeastern Spain. Genomic DNA samples of 113 C. parvum isolates from diarrheic pre-weaned lambs collected in 49 meat-type sheep farms were analyzed. Loci exhibited various degrees of polymorphism, the finding of 7–9 alleles in the four most variable and discriminatory markers (ML2, Cgd6_5400, Cgd6_3940, and GP60) being remarkable. The combination of alleles at the twelve loci identified a total of 74 multilocus subtypes (MLTs) and provided a Hunter-Gaston discriminatory index of 0.988 (95% CI, 0.979-0.996). The finding that most MLTs (n = 64) were unique to individual farms evidenced that cryptosporidial infection is mainly transmitted within sheep flocks, with herd-to-herd transmission playing a secondary role. Limited intra- host variability was found, since only five isolates were genotypically mixed. In contrast, a significant intra-farm genetic diversity was seen, with the presence of multiple MLTs on more than a half of the farms (28/46), suggesting frequent mutations or genetic exchange through recombination. Comparison with a previous study in calves in northern Spain using the same 12-loci typing approach showed differences in the identity of major alleles at most loci, with a single MLT being shared between lambs and calves. Analysis of evolutionary descent by the algorithm eBURST indicated a high degree of genetic divergence, with over 41% MLTs appearing as singletons along with a high number of clonal complexes, most of them linking only two MLTs. Bayesian Structure analysis and F statistics also revealed the genetic remoteness of most C. parvum isolates and no ancestral population size was chosen. Linkage analysis evidenced a prevalent pattern of clonality within the parasite population.
000048693 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B82$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32138
000048693 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000048693 590__ $$a2.806$$b2016
000048693 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000048693 592__ $$a1.236$$b2016
000048693 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000048693 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000048693 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000048693 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000048693 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, L.V.$$uUniversidad de Zaragoza
000048693 700__ $$0(orcid)0000-0002-9453-7655$$aDel Cacho, E.$$uUniversidad de Zaragoza
000048693 700__ $$0(orcid)0000-0002-3532-0384$$aSánchez-Acedo, C.$$uUniversidad de Zaragoza
000048693 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, J.$$uUniversidad de Zaragoza
000048693 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000048693 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000048693 773__ $$g11, 5 (2016), 0155336 [16 p.]$$pPLoS One$$tPloS one$$x1932-6203
000048693 8564_ $$s628903$$uhttps://zaguan.unizar.es/record/48693/files/texto_completo.pdf$$yVersión publicada
000048693 8564_ $$s103682$$uhttps://zaguan.unizar.es/record/48693/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000048693 909CO $$ooai:zaguan.unizar.es:48693$$particulos$$pdriver
000048693 951__ $$a2020-02-21-13:08:43
000048693 980__ $$aARTICLE