doi:10.3390/molecules21040449engFerruz, E.Atanasova-Pénichon, V.Bonnin-Verdal, M.Marchegay, G.Pinson-Gadais, L.Ducos, C.Lorán, S.Ariño, A.Barreau, C.Richard-Forget, F.Effects of Phenolic Acids on the Growth and Production of T-2 and HT-2 Toxins by Fusarium langsethiae and F. sporotrichioidesART-2016-94958The effect of natural phenolic acids was tested on the growth and production of T-2 and HT-2 toxins by Fusarium langsethiae and F. sporotrichioides, on Mycotoxin Synthetic medium. Plates treated with 0.5 mM of each phenolic acid (caffeic, chlorogenic, ferulic and p-coumaric) and controls without phenolic acid were incubated for 14 days at 25 °C. Fungal biomass of F. langsethiae and F. sporotrichioides was not reduced by the phenolic acids. However, biosynthesis of T-2 toxin by F. langsethiae was significantly reduced by chlorogenic (23.1%) and ferulic (26.5%) acids. Production of T-2 by F. sporotrichioides also decreased with ferulic acid by 23% (p < 0.05). In contrast, p-coumaric acid significantly stimulated the production of T-2 and HT-2 toxins for both strains. A kinetic study of F. langsethiae with 1 mM ferulic acid showed a significant decrease in fungal biomass, whereas T-2 production increased after 10 days of incubation. The study of gene expression in ferulic supplemented cultures of F. langsethiae revealed a significant inhibition for Tri5, Tri6 and Tri12 genes, while for Tri16 the decrease in gene expression was not statistically significant. Overall, results indicated that phenolic acids had a variable effect on fungal growth and mycotoxin production, depending on the strain and the concentration and type of phenolic acid assayed.2016http://zaguan.unizar.es/record/5606010.3390/molecules21040449http://zaguan.unizar.es/record/56060oai:zaguan.unizar.es:56060info:eu-repo/grantAgreement/ES/DGA/A01info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2011-26808info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2014-52648-REDTinfo:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2014-57069-RMolecules 21, 4 (2016), 449 [11 pp]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess