56060 20200221144314.0 doi 10.3390/molecules21040449 sideral 94958 ART-2016-94958 eng (orcid)0000-0001-8734-1446 Ferruz, E. Effects of Phenolic Acids on the Growth and Production of T-2 and HT-2 Toxins by Fusarium langsethiae and F. sporotrichioides 2016 Access copy available to the general public Unrestricted The effect of natural phenolic acids was tested on the growth and production of T-2 and HT-2 toxins by Fusarium langsethiae and F. sporotrichioides, on Mycotoxin Synthetic medium. Plates treated with 0.5 mM of each phenolic acid (caffeic, chlorogenic, ferulic and p-coumaric) and controls without phenolic acid were incubated for 14 days at 25 °C. Fungal biomass of F. langsethiae and F. sporotrichioides was not reduced by the phenolic acids. However, biosynthesis of T-2 toxin by F. langsethiae was significantly reduced by chlorogenic (23.1%) and ferulic (26.5%) acids. Production of T-2 by F. sporotrichioides also decreased with ferulic acid by 23% (p < 0.05). In contrast, p-coumaric acid significantly stimulated the production of T-2 and HT-2 toxins for both strains. A kinetic study of F. langsethiae with 1 mM ferulic acid showed a significant decrease in fungal biomass, whereas T-2 production increased after 10 days of incubation. The study of gene expression in ferulic supplemented cultures of F. langsethiae revealed a significant inhibition for Tri5, Tri6 and Tri12 genes, while for Tri16 the decrease in gene expression was not statistically significant. Overall, results indicated that phenolic acids had a variable effect on fungal growth and mycotoxin production, depending on the strain and the concentration and type of phenolic acid assayed. info:eu-repo/grantAgreement/ES/DGA/A01 info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2011-26808 info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2014-52648-REDT info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2014-57069-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.861 2016 CHEMISTRY, ORGANIC 17 / 59 = 0.288 2016 Q2 T1 0.824 2016 Analytical Chemistry 2016 Q1 Pharmaceutical Science 2016 Q1 Chemistry (miscellaneous) 2016 Q1 Organic Chemistry 2016 Q2 Physical and Theoretical Chemistry 2016 Q2 Drug Discovery 2016 Q2 Medicine (miscellaneous) 2016 Q2 Molecular Medicine 2016 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Atanasova-Pénichon, V. Bonnin-Verdal, M. Marchegay, G. Pinson-Gadais, L. Ducos, C. (orcid)0000-0002-1961-8551 Lorán, S. Universidad de Zaragoza (orcid)0000-0001-6325-7100 Ariño, A. Universidad de Zaragoza Barreau, C. Richard-Forget, F. 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 21, 4 (2016), 449 [11 pp] Molecules Molecules 1420-3049 1142149 http://zaguan.unizar.es/record/56060/files/texto_completo.pdf Versión publicada 105948 http://zaguan.unizar.es/record/56060/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:56060 articulos driver 2020-02-21-13:38:51 ARTICLE