56067 20200221144250.0 doi 10.1021/jacs.5b13577 sideral 95015 ART-2016-95015 eng Pedersen, J.T. Amyloid-ß and a-Synuclein Decrease the Level of Metal-Catalyzed Reactive Oxygen Species by Radical Scavenging and Redox Silencing 2016 Access copy available to the general public Unrestricted The formation of reactive oxygen species (ROS) is linked to the pathogenesis of neurodegenerative diseases. Here we have investigated the effect of soluble and aggregated amyloid-ß (Aß) and a-synuclein (aS), associated with Alzheimer''s and Parkinson''s diseases, respectively, on the Cu2+-catalyzed formation of ROS in vitro in the presence of a biological reductant. We find that the levels of ROS, and the rate by which ROS is generated, are significantly reduced when Cu2+ is bound to Aß or aS, particularly when they are in their oligomeric or fibrillar forms. This effect is attributed to a combination of radical scavenging and redox silencing mechanisms. Our findings suggest that the increase in ROS associated with the accumulation of aggregated Aß or aS does not result from a particularly ROS-active form of these peptides, but rather from either a local increase of Cu2+ and other ROS-active metal ions in the aggregates or as a downstream consequence of the formation of the pathological amyloid structures. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 13.858 2016 CHEMISTRY, MULTIDISCIPLINARY 10 / 166 = 0.06 2016 Q1 T1 7.492 2016 Biochemistry 2016 Q1 Colloid and Surface Chemistry 2016 Q1 Chemistry (miscellaneous) 2016 Q1 Catalysis 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Chen, S.W. Borg, C.B. Ness, S. Bahl, J.M. Heegaard, N.H.H. Dobson, C.M. Hemmingsen, L. (orcid)0000-0002-9138-6687 Cremades, N. Universidad de Zaragoza Teilum, K. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 138, 12 (2016), 3966-3969 J. Am. Chem. Soc. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 0002-7863 1085384 http://zaguan.unizar.es/record/56067/files/texto_completo.pdf Versión publicada 131738 http://zaguan.unizar.es/record/56067/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:56067 articulos driver 2020-02-21-13:27:07 ARTICLE