56081 20200221144200.0 doi 10.1371/journal.pone.0157967 sideral 95096 ART-2016-95096 eng (orcid)0000-0002-6305-9283 Manzano, Sara Inhomogeneous response of articular cartilage: A three-dimensional multiphasic heterogeneous study 2016 Access copy available to the general public Unrestricted Articular cartilage exhibits complex mechano-electrochemical behaviour due to its anisotropy, inhomogeneity and material non-linearity. In this work, the thickness and radial dependence of cartilage properties are incorporated into a 3D mechano-electrochemical model to explore the relevance of heterogeneity in the behaviour of the tissue. The model considers four essential phenomena: (i) osmotic pressure, (ii) convective and diffusive processes, (iii) chemical expansion and (iv) three-dimensional through-the-thickness heterogeneity of the tissue. The need to consider heterogeneity in computational simulations of cartilage behaviour and in manufacturing biomaterials mimicking this tissue is discussed. To this end, healthy tibial plateaus from pigs were mechanically and biochemically tested in-vitro. Heterogeneous properties were included in the mechano-electrochemical computational model to simulate tissue swelling. The simulation results demonstrated that swelling of the heterogeneous samples was significantly lower than swelling under homogeneous and isotropic conditions. Furthermore, there was a significant reduction in the flux of water and ions in the former samples. In conclusion, the computational model presented here can be con- sidered as a valuable tool for predicting how the variation of cartilage properties affects its behaviour, opening up possibilities for exploring the requirements of cartilage-mimicking biomaterials for tissue engineering. Besides, the model also allows the establishment of behavioural patterns of swelling and of water and ion fluxes in articular cartilage. info:eu-repo/grantAgreement/ES/MEC/FPU-AP2010-2557 info:eu-repo/grantAgreement/ES/MINECO/MAT2013-46467-C4-3-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.806 2016 MULTIDISCIPLINARY SCIENCES 15 / 63 = 0.238 2016 Q1 T1 1.236 2016 Agricultural and Biological Sciences (miscellaneous) 2016 Q1 Medicine (miscellaneous) 2016 Q1 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Armengol, Monica J.Price, Andrew A.Hulley, Philippa S.Gill, Harinderjit (orcid)0000-0001-8741-6452 Doblaré, Manuel Universidad de Zaragoza (orcid)0000-0003-0088-7222 Hamdy Doweidar, Mohamed Universidad de Zaragoza 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 11, 6 (2016), 0157967 [19p.] PLoS One PloS one 1932-6203 13146029 http://zaguan.unizar.es/record/56081/files/texto_completo.pdf Versión publicada 103620 http://zaguan.unizar.es/record/56081/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:56081 articulos driver 2020-02-21-13:08:50 ARTICLE