000056098 001__ 56098
000056098 005__ 20200221144133.0
000056098 0247_ $$2doi$$a10.1039/c5nj02775f
000056098 0248_ $$2sideral$$a93659
000056098 037__ $$aART-2016-93659
000056098 041__ $$aeng
000056098 100__ $$aMaicas Gabas, I.
000056098 245__ $$aIn vitro cell cytotoxicity profile and morphological response to polyoxometalate-stabilised gold nanoparticles
000056098 260__ $$c2016
000056098 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056098 5203_ $$aThe size and redox properties of molecular polyoxometalates (POMs) make them extremely relevant for bioapplications: from disrupting tumour growth and enzyme inhibition, to DNA-intercalating agents and antimicrobial applications. Their unique ability to reversibly dominate and receive electrons, coupled with their high anionic charge, also makes them suitable for the preparation of zero-valent state metal nanoparticles (NPs) from molecular precursors. Polyoxometalate-stabilised nanoparticles (NPs@POM) are therefore an ideal delivery vehicle for bioactive POMs. Here we show how POM-stabilised gold NPs (AuNPs@POM) are massively internalised into Vero (kidney epithelial) and B16 (skin melanoma) cell lines with variable cytotoxic effects. Cell viability assays and quantification of cytoplasmic membrane composition revealed that the Vero cell line was unaltered by the internalisation of these hybrid particles; while their internalisation in B16 tumour cells produced mild cytotoxic effects and an antiproliferative cell cycle arrest in the G0/G1 and G2/M phases. The observed perturbation of the tumour cell line combined with the high degree of internalisation means that these (or similar) NPs@POM could serve as candidates for a range of bioapplications in diagnostics or therapy.
000056098 536__ $$9info:eu-repo/grantAgreement/EC/FP7/239931/EU/Multifunctional Magnetic Nanoparticles: Towards Smart Drugs Design/NANOPUZZLE
000056098 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000056098 590__ $$a3.269$$b2016
000056098 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 166 = 0.313$$c2016$$dQ2$$eT1
000056098 592__ $$a0.868$$b2016
000056098 593__ $$aChemistry (miscellaneous)$$c2016$$dQ1
000056098 593__ $$aMaterials Chemistry$$c2016$$dQ1
000056098 593__ $$aCatalysis$$c2016$$dQ2
000056098 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056098 700__ $$0(orcid)0000-0002-4170-7999$$aStepien, G.
000056098 700__ $$0(orcid)0000-0002-2861-2469$$aMoros, M.
000056098 700__ $$0(orcid)0000-0003-4848-414X$$aMitchell, S.G.
000056098 700__ $$0(orcid)0000-0003-1081-8482$$aMartinez de la Fuente, J.$$uUniversidad de Zaragoza
000056098 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000056098 773__ $$g40, 2 (2016), 1039-1047$$pNew j. chem.$$tNEW JOURNAL OF CHEMISTRY$$x1144-0546
000056098 8564_ $$s2347077$$uhttps://zaguan.unizar.es/record/56098/files/texto_completo.pdf$$yVersión publicada
000056098 8564_ $$s114884$$uhttps://zaguan.unizar.es/record/56098/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056098 909CO $$ooai:zaguan.unizar.es:56098$$particulos$$pdriver
000056098 951__ $$a2020-02-21-13:04:51
000056098 980__ $$aARTICLE