56165 20200221144312.0 doi 10.3390/ijms17060964 sideral 95298 ART-2016-95298 eng López, L.C. Benzbromarone, quercetin, and folic acid inhibit amylin aggregation 2016 Access copy available to the general public Unrestricted Human Amylin, or islet amyloid polypeptide (hIAPP), is a small hormone secreted by pancreatic ß-cells that forms aggregates under insulin deficiency metabolic conditions, and it constitutes a pathological hallmark of type II diabetes mellitus. In type II diabetes patients, amylin is abnormally increased, self-assembled into amyloid aggregates, and ultimately contributes to the apoptotic death of ß-cells by mechanisms that are not completely understood. We have screened a library of approved drugs in order to identify inhibitors of amylin aggregation that could be used as tools to investigate the role of amylin aggregation in type II diabetes or as therapeutics in order to reduce ß-cell damage. Interestingly, three of the compounds analyzed—benzbromarone, quercetin, and folic acid—are able to slow down amylin fiber formation according to Thioflavin T binding, turbidimetry, and Transmission Electron Microscopy assays. In addition to the in vitro assays, we have tested the effect of these compounds in an amyloid toxicity cell culture model and we have found that one of them, quercetin, has the ability to partly protect cultured pancreatic insulinoma cells from the cytotoxic effect of amylin. Our data suggests that quercetin can contribute to reduce oxidative damage in pancreatic insulinoma ß cells by modulating the aggregation propensity of amylin. info:eu-repo/grantAgreement/ES/MICINN/BFU2013-44763-P info:eu-repo/grantAgreement/ES/MINECO/BFU2013-47064-P info:eu-repo/grantAgreement/ES/UZ/UZ2011-BIO-02 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.226 2016 CHEMISTRY, MULTIDISCIPLINARY 54 / 166 = 0.325 2016 Q2 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 116 / 287 = 0.404 2016 Q2 T2 1.235 2016 Medicine (miscellaneous) 2016 Q1 Physical and Theoretical Chemistry 2016 Q1 Computer Science Applications 2016 Q1 Inorganic Chemistry 2016 Q1 Spectroscopy 2016 Q1 Organic Chemistry 2016 Q1 Molecular Biology 2016 Q2 Catalysis 2016 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Varea, O. Navarro, S. (orcid)0000-0003-0062-1029 Carrodeguas, J.A. Universidad de Zaragoza De Groot, N.S. Ventura, S. (orcid)0000-0002-2879-9200 Sancho, J. Universidad de Zaragoza 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 17, 6 (2016), 964 [12 pp] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 2215331 http://zaguan.unizar.es/record/56165/files/texto_completo.pdf Versión publicada 105516 http://zaguan.unizar.es/record/56165/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:56165 articulos driver 2020-02-21-13:37:36 ARTICLE