000056206 001__ 56206
000056206 005__ 20200221144246.0
000056206 0247_ $$2doi$$a10.1371/journal.pone.0150432
000056206 0248_ $$2sideral$$a94517
000056206 037__ $$aART-2016-94517
000056206 041__ $$aeng
000056206 100__ $$aNymo, Ingebjørg H.
000056206 245__ $$aMarine Mammal Brucella Reference Strains are Attenuated in a BALB/c Mouse Model.
000056206 260__ $$c2016
000056206 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056206 5203_ $$aBrucellosis is a zoonosis of worldwide distribution with numerous animal host species. Since the novel isolation of Brucella spp. from marine mammals in 1994 the bacteria have been isolated from various marine mammal hosts. The marine mammal reference strains Brucella pinnipedialis 12890 (harbour seal, Phoca vitulina) and Brucella ceti 12891 (harbour porpoise, Phocoena phocoena) were included in genus Brucella in 2007, however, their pathogenicity in the mouse model is pending. Herein this is evaluated in BALB/c mice with Brucella suis 1330 as a control. Both marine mammal strains were attenuated, however, B. ceti was present at higher levels than B. pinnipedialis in blood, spleen and liver throughout the infection, in addition B. suis and B. ceti were isolated from brains and faeces at times with high levels of bacteraemia. In B. suis-infected mice serum cytokines peaked at day 7. In B. pinnipedialis-infected mice, levels were similar, but peaked predominantly at day 3 and an earlier peak in spleen weight likewise implied an earlier response. The inflammatory response induced pathology in the spleen and liver. In B. ceti-infected mice, most serum cytokine levels were comparable to those in uninfected mice, consistent with a limited inflammatory response, which also was indicated by restricted spleen and liver pathology. Specific immune responses against all three strains were detected in vitro after stimulation of splenocytes from infected mice with the homologous heat-killed brucellae. Antibody responses in vivo were also induced by the three brucellae. The immunological pattern of B. ceti in combination with persistence in organs and limited pathology has heretofore not been described for other brucellae. These two marine mammal wildtype strains show an attenuated pattern in BALB/c mice only previously described for Brucella neotomea.
000056206 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R$$9info:eu-repo/grantAgreement/ES/INIA/RTA2013-00065-C02-01
000056206 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000056206 590__ $$a2.806$$b2016
000056206 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000056206 592__ $$a1.236$$b2016
000056206 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000056206 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000056206 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000056206 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056206 700__ $$0(orcid)0000-0002-9730-2210$$aArias, Maykel A.
000056206 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, Julian$$uUniversidad de Zaragoza
000056206 700__ $$aAlvarez, María Pilar
000056206 700__ $$aAlcaraz, Ana
000056206 700__ $$aGodfroid, Jacquers
000056206 700__ $$0(orcid)0000-0002-5490-5177$$aJimenez de Bagüés, María Pilar
000056206 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología
000056206 773__ $$g11, 3 (2016), 0150432 [19 p]$$pPLoS One$$tPloS one$$x1932-6203
000056206 8564_ $$s849060$$uhttps://zaguan.unizar.es/record/56206/files/texto_completo.pdf$$yVersión publicada
000056206 8564_ $$s105740$$uhttps://zaguan.unizar.es/record/56206/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056206 909CO $$ooai:zaguan.unizar.es:56206$$particulos$$pdriver
000056206 951__ $$a2020-02-21-13:25:13
000056206 980__ $$aARTICLE