56248 20241129133040.0 doi 10.3390/molecules200611357 sideral 91409 ART-2015-91409 eng Espina, L. (orcid)0000-0002-3170-9755 Individual constituents from essential oils inhibit biofilm mass production by multi-drug resistant staphylococcus aureus 2015 Access copy available to the general public Unrestricted Biofilm formation by Staphylococcus aureus represents a problem in both the medical field and the food industry, because the biofilm structure provides protection to embedded cells and it strongly attaches to surfaces. This circumstance is leading to many research programs seeking new alternatives to control biofilm formation by this pathogen. In this study we show that a potent inhibition of biofilm mass production can be achieved in community-associated methicillin-resistant S. aureus (CA-MRSA) and methicillin-sensitive strains using plant compounds, such as individual constituents (ICs) of essential oils (carvacrol, citral, and (+)-limonene). The Crystal Violet staining technique was used to evaluate biofilm mass formation during 40 h of incubation. Carvacrol is the most effective IC, abrogating biofilm formation in all strains tested, while CA-MRSA was the most sensitive phenotype to any of the ICs tested. Inhibition of planktonic cells by ICs during initial growth stages could partially explain the inhibition of biofilm formation. Overall, our results show the potential of EOs to prevent biofilm formation, especially in strains that exhibit resistance to other antimicrobials. As these compounds are food additives generally recognized as safe, their anti-biofilm properties may lead to important new applications, such as sanitizers, in the food industry or in clinical settings. info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.465 2015 CHEMISTRY, ORGANIC 24 / 59 = 0.407 2015 Q2 T2 0.57 2015 Analytical Chemistry 2015 Q2 Chemistry (miscellaneous) 2015 Q2 Drug Discovery 2015 Q2 Physical and Theoretical Chemistry 2015 Q2 Medicine (miscellaneous) 2015 Q2 Organic Chemistry 2015 Q2 Pharmaceutical Science 2015 Q2 Molecular Medicine 2015 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pagán, R. Universidad de Zaragoza (orcid)0000-0002-0238-6328 López, D. García-Gonzalo, D. Universidad de Zaragoza (orcid)0000-0002-7629-8101 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 20, 6 (2015), 11357-11372 Molecules (Basel, Online) Molecules 1420-3049 1034894 http://zaguan.unizar.es/record/56248/files/texto_completo.pdf Versión publicada 93968 http://zaguan.unizar.es/record/56248/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:56248 articulos driver 2024-11-29-13:28:55 ARTICLE