000056267 001__ 56267
000056267 005__ 20210121114531.0
000056267 0247_ $$2doi$$a10.1186/s12864-015-1703-1
000056267 0248_ $$2sideral$$a91706
000056267 037__ $$aART-2015-91706
000056267 041__ $$aeng
000056267 100__ $$aYingping, F.
000056267 245__ $$aThe Pkn22 Ser/Thr kinase in Nostoc PCC 7120: Role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress
000056267 260__ $$c2015
000056267 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056267 5203_ $$aBackground: The filamentous cyanobacterium Nostoc sp. strain PCC 7120 can fix N2 when combined nitrogen is not available. Furthermore, it has to cope with reactive oxygen species generated as byproducts of photosynthesis and respiration. We have previously demonstrated the synthesis of Ser/Thr kinase Pkn22 as an important survival response of Nostoc to oxidative damage. In this study we wished to investigate the possible involvement of this kinase in signalling peroxide stress and nitrogen deprivation.
Results: Quantitative RT-PCR experiments revealed that the pkn22 gene is induced in response to peroxide stress and to combined nitrogen starvation. Electrophoretic motility assays indicated that the pkn22 promoter is recognized by the global transcriptional regulators FurA and NtcA. Transcriptomic analysis comparing a pkn22-insertion mutant and the wild type strain indicated that this kinase regulates genes involved in important cellular functions such as photosynthesis, carbon metabolism and iron acquisition. Since metabolic changes may lead to oxidative stress, we investigated whether this is the case with nitrogen starvation. Our results rather invalidate this hypothesis thereby suggesting that the function of Pkn22 under nitrogen starvation is independent of its role in response to peroxide stress.
Conclusions: Our analyses have permitted a more complete functional description of Ser/Thr kinase in Nostoc. We have decrypted the transcriptional regulation of the pkn22 gene, and analysed the whole set of genes under the control of this kinase in response to the two environmental changes often encountered by cyanobacteria in their natural habitat: oxidative stress and nitrogen deprivation.
000056267 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000056267 590__ $$a3.867$$b2015
000056267 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b32 / 161 = 0.199$$c2015$$dQ1$$eT1
000056267 591__ $$aGENETICS & HEREDITY$$b43 / 166 = 0.259$$c2015$$dQ2$$eT1
000056267 592__ $$a2.348$$b2015
000056267 593__ $$aGenetics$$c2015$$dQ1
000056267 593__ $$aBiotechnology$$c2015$$dQ1
000056267 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056267 700__ $$aLemeille, S.
000056267 700__ $$0(orcid)0000-0002-0531-0943$$aGonzález, A.$$uUniversidad de Zaragoza
000056267 700__ $$aRisoul, V.
000056267 700__ $$aDenis, Y.
000056267 700__ $$aRichaud, P.
000056267 700__ $$aLamrabet, O.
000056267 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, M.F.$$uUniversidad de Zaragoza
000056267 700__ $$aZhang, C.C
000056267 700__ $$aLatifi, A.
000056267 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000056267 773__ $$g16, 557 (2015), [11 pp.]$$pBMC genomics$$tBMC Genomics$$x1471-2164
000056267 8564_ $$s1692566$$uhttps://zaguan.unizar.es/record/56267/files/texto_completo.pdf$$yVersión publicada
000056267 8564_ $$s105751$$uhttps://zaguan.unizar.es/record/56267/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056267 909CO $$ooai:zaguan.unizar.es:56267$$particulos$$pdriver
000056267 951__ $$a2021-01-21-11:10:28
000056267 980__ $$aARTICLE