000056324 001__ 56324
000056324 005__ 20200221144348.0
000056324 0247_ $$2doi$$a10.3835/plantgenome2015.10.0101
000056324 0248_ $$2sideral$$a95626
000056324 037__ $$aART-2016-95626
000056324 041__ $$aeng
000056324 100__ $$aCantalapiedra, C.P.
000056324 245__ $$aA cluster of nucleotide-binding site-leucine-rich repeat genes resides in a barley powdery mildew resistance quantitative trait loci on 7HL
000056324 260__ $$c2016
000056324 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056324 5203_ $$aPowdery mildew causes severe yield losses in barley production worldwide. Although many resistance genes have been described, only a few have already been cloned. A strong QTL (quantitative trait locus) conferring resistance to a wide array of powdery mildew isolates was identified in a Spanish barley landrace on the long arm of chromosome 7H. Previous studies narrowed down the QTL position, but were unable to identify candidate genes or physically locate the resistance. In this study, the exome of three recombinant lines from a high-resolution mapping population was sequenced and analyzed, narrowing the position of the resistance down to a single physical contig. Closer inspection of the region revealed a cluster of closely related NBS-LRR (nucleotide-binding site–leucine-rich repeat containing protein) genes. Large differences were found between the resistant lines and the reference genome of cultivar Morex, in the form of PAV (presence-absence variation) in the composition of the NBS-LRR cluster. Finally, a template-guided assembly was performed and subsequent expression analysis revealed that one of the new assembled candidate genes is transcribed. In summary, the results suggest that NBS-LRR genes, absent from the reference and the susceptible genotypes, could be functional and responsible for the powdery mildew resistance. The procedure followed is an example of the use of NGS (next-generation sequencing) tools to tackle the challenges of gene cloning when the target gene is absent from the reference genome.
000056324 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/RFP2012-00015-00-00$$9info:eu-repo/grantAgreement/ES/MINECO/BES-2011-045905$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2013-487569$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2010-21929
000056324 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000056324 590__ $$a2.736$$b2016
000056324 591__ $$aPLANT SCIENCES$$b48 / 211 = 0.227$$c2016$$dQ1$$eT1
000056324 591__ $$aGENETICS & HEREDITY$$b78 / 166 = 0.47$$c2016$$dQ2$$eT2
000056324 592__ $$a1.611$$b2016
000056324 593__ $$aAgronomy and Crop Science$$c2016$$dQ1
000056324 593__ $$aPlant Science$$c2016$$dQ1
000056324 593__ $$aGenetics$$c2016$$dQ2
000056324 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056324 700__ $$0(orcid)0000-0002-5462-907X$$aContreras-Moreira, B.$$uUniversidad de Zaragoza
000056324 700__ $$aSilvar, C.
000056324 700__ $$aPerovic, D.
000056324 700__ $$aOrdon, F.
000056324 700__ $$aGracia, M.P.
000056324 700__ $$aIgartua, E.
000056324 700__ $$aCasas, A.M.
000056324 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000056324 773__ $$g9, 2 (2016), [14 pp.]$$pPlant genome$$tPlant genome$$x1940-3372
000056324 8564_ $$s1807256$$uhttps://zaguan.unizar.es/record/56324/files/texto_completo.pdf$$yVersión publicada
000056324 8564_ $$s99944$$uhttps://zaguan.unizar.es/record/56324/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056324 909CO $$ooai:zaguan.unizar.es:56324$$particulos$$pdriver
000056324 951__ $$a2020-02-21-13:53:39
000056324 980__ $$aARTICLE