56358 20210121114445.0 doi 10.1007/s00216-015-8959-9 sideral 92282 ART-2015-92282 eng (orcid)0000-0002-8934-3979 Gracia-Moreno, E. Quantitative determination of five hydroxy acids, precursors of relevant wine aroma compounds in wine and other alcoholic beverages 2015 Access copy available to the general public Unrestricted A method for the quantitative determination of 2-hydroxy-2-methylbutanoic (2OH2MB), 2-hydroxy-3-methylbutanoic (2OH3MB), 3-hydroxy-3-methylbutanoic (3OH3MB), 2-hydroxy-4-methylpentanoic (2OH4MP) and 3-hydroxybutanoic (3OHB) acids has been optimized, validated and applied to a set of wines and other alcoholic beverages. The analytes were preconcentrated in a solid phase extraction cartridge and derivatized with 2, 3, 4, 5, 6-pentafluorobenzyl bromide at room temperature for 30 min, followed by GC-MS analysis. Detection limits were between 0.5 and 29 µg L-1, and linearity was maintained up to 3 or 12 mg L-1, depending on the analyte. Recovery values were between 85 and 106 %, and reproducibility was better than 12 % RSD in most cases. The first specific study of these analytes in wine and other alcoholic beverages is herein reported. Concentrations ranged from the method detection limits to 7820, 519, 8510, 3470 and 2500 µg L-1 for 2OH2MB, 2OH3MB, 3OH3MB, 2OH4MP and 3OHB, respectively, which may have relevant sensory effects. These products were not found in distillates (except 3OHB) but were all present in beer. 2OH2MB, 3OH3MB and 3OHB were found in unfermented grape derivatives. Sherry wines had the highest levels of all except for 3OHB. info:eu-repo/grantAgreement/ES/CICYT/AGL2010-22355-C02-01 info:eu-repo/grantAgreement/ES/MEC/FPU-AP2008-03811 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.125 2015 CHEMISTRY, ANALYTICAL 15 / 75 = 0.2 2015 Q1 T1 BIOCHEMICAL RESEARCH METHODS 25 / 77 = 0.325 2015 Q2 T1 1.059 2015 Analytical Chemistry 2015 Q1 Biochemistry 2015 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-0730-6606 Lopez, R. Universidad de Zaragoza (orcid)0000-0002-4353-2483 Ferreira, V. Universidad de Zaragoza 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 407, 26 (2015), 7925-7934 Anal. Bioanal. Chem. Analytical and Bioanalytical Chemistry 1618-2642 2792501 http://zaguan.unizar.es/record/56358/files/texto_completo.pdf Postprint 86214 http://zaguan.unizar.es/record/56358/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:56358 articulos driver 2021-01-21-10:42:38 ARTICLE