56747 20200221144219.0 doi 10.3390/molecules21070843 sideral 96124 ART-2016-96124 eng Stojkovic, S. Resistance to DNA Damaging agents produced invasive phenotype of rat glioma cells-characterization of a new in vivo model 2016 Access copy available to the general public Unrestricted Chemoresistance and invasion properties are severe limitations to efficient glioma therapy. Therefore, development of glioma in vivo models that more accurately resemble the situation observed in patients emerges. Previously, we established RC6 rat glioma cell line resistant to DNA damaging agents including antiglioma approved therapies such as 3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and temozolomide (TMZ). Herein, we evaluated the invasiveness of RC6 cells in vitro and in a new orthotopic animal model. For comparison, we used C6 cells from which RC6 cells originated. Differences in cell growth properties were assessed by real-time cell analyzer. Cells’ invasive potential in vitro was studied in fluorescently labeled gelatin and by formation of multicellular spheroids in hydrogel. For animal studies, fluorescently labeled cells were inoculated into adult male Wistar rat brains. Consecutive coronal and sagittal brain sections were analyzed 10 and 25 days post-inoculation, while rats’ behavior was recorded during three days in the open field test starting from 25th day post-inoculation. We demonstrated that development of chemoresistance induced invasive phenotype of RC6 cells with significant behavioral impediments implying usefulness of orthotopic RC6 glioma allograft in preclinical studies for the examination of new approaches to counteract both chemoresistance and invasion of glioma cells. info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R info:eu-repo/grantAgreement/ES/MINECO/MTM2015-71200-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.861 2016 CHEMISTRY, ORGANIC 17 / 59 = 0.288 2016 Q2 T1 0.824 2016 Analytical Chemistry 2016 Q1 Pharmaceutical Science 2016 Q1 Chemistry (miscellaneous) 2016 Q1 Organic Chemistry 2016 Q2 Physical and Theoretical Chemistry 2016 Q2 Drug Discovery 2016 Q2 Medicine (miscellaneous) 2016 Q2 Molecular Medicine 2016 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Podolski-Renic, A. Dinic, J. Pavkovic, Ž. (orcid)0000-0002-9414-1845 Ayuso, J.M. Universidad de Zaragoza Fernández, L.J. (orcid)0000-0003-2410-5678 Ochoa, I. Universidad de Zaragoza Pérez-García, V.M. Pešic, V. Pešic, M. 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 21, 7 (2016), 21070843[16 p.] Molecules Molecules 1420-3049 8889233 http://zaguan.unizar.es/record/56747/files/texto_completo.pdf Versión publicada 105336 http://zaguan.unizar.es/record/56747/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:56747 articulos driver 2020-02-21-13:13:46 ARTICLE