56831 20210121114540.0 doi 10.14306/renhyd.19.1.110 sideral 89481 ART-2015-89481 eng (orcid)0000-0002-1886-9586 Menal-Puey Susana Universidad de Zaragoza Validation of a rapid method for detecting nitrate in chard (Beta vulgaris cycla). Analysis of spanish commercial samples marketed in the region of huesca, spain, and estimation of the daily intake 2015 Access copy available to the general public Unrestricted Introduction: This paper presents the validation of a rapid method for the determination of nitrate content in chard samples, in order to determine, during two periods (winter and summer of 2012), the current levels of nitrate in this typical vegetable, and the toxicological risk associated with this intake. Material and Methods: A rapid colorimetric determination of nitrate in chard samples by nitration of salicylic acid was validated. The validated method was applied to analyze the content of nitrate in 56 chard samples marketed in Huesca (Spain) and collected in winter and summer seasons, and the toxicological risk associated with the intake for adult and children population was evaluated. Results: The method was specific and robust enough for the required purposes. The main performance characteristics of the method were: limits of detection and quantitation of 0.29 mg L-1 and 0.59 mg L-1, respectively; recoveries from 80.0% to 107.4%; and coefficients of variation lower than 11.4%. The detected mean nitrate content was 2293 mg kg-1 and there was evidence of risk only for extreme consumers (adults and children), especially in winter period. Conclusions: A high percentage of chard samples with a considerable concentration of nitrate were found. Taking into account the estimated dairy intake of nitrate associated with them, it could be recommended to establish a regulatory limit of nitrate to chard, a vegetable of important consumption in Spain. info:eu-repo/grantAgreement/ES/DGA/T10 info:eu-repo/grantAgreement/ES/DGA/A01 info:eu-repo/semantics/openAccess by-nc-sa http://creativecommons.org/licenses/by-nc-sa/3.0/es/ 0.122 2015 Nutrition and Dietetics 2015 Q4 Food Science 2015 Q4 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0001-8822-7303 Asensio Casas, Esther Universidad de Zaragoza 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 19, 1 (2015), 4-11 Rev. esp. nutr. hum. diet. Revista española de nutrición humana y dietética 2173-1292 957935 http://zaguan.unizar.es/record/56831/files/texto_completo.pdf Versión publicada 81503 http://zaguan.unizar.es/record/56831/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:56831 articulos driver 2021-01-21-11:15:40 ARTICLE