57068 20200813075837.0 doi 10.1016/j.jevs.2016.08.002 sideral 96193 ART-2016-96193 eng (orcid)0000-0003-4071-1467 Luesma, María José Universidad de Zaragoza Primary cilia in chondrogenic differentiation of equine bone marrow mesenchymal stem cells: ultrastructural study 2016 Access copy available to the general public Unrestricted Locomotor system disorders in equine species, such as tendon lesions, osteoarthritis, or ligament injuries, are some of the most frequent causes of dramatic reduction in horse performance. Traditional therapies are aimed at the inflammatory process and pain, but they do not regenerate normal tendon or ligament matrix and do not reduce re-injured rates. Mesenchymal stem cells have started to use as therapeutic option to repair these injured tissues. Most studies have focused on their isolation, in vitro culture and pheno- typing. However, mesenchymal stem cell ultrastructure has been disregarded in the last years. We investigate the ultrastructural characteristics of these cells once differentiated into chondrocytes. Ultrastructural analysis was conducted on suspension cultures of differentiated chondrocytes from bone marrow mesenchymal stem cells by means of transmission electron microscopy. The morphologic characteristics of these cells, their ability to produce the extracellular matrix, and the presence of a single cilium could be indicative of the mesenchymal cells differentiation into chondrocyte phenotype. This study provides essential data to evaluate the degree of suitable phenotypic stability, for these cells can be used with repair purposes. info:eu-repo/grantAgreement/ES/UZ/JIUZ-2013-FIS-04 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 0.882 2016 VETERINARY SCIENCES 68 / 136 = 0.5 2016 Q2 T2 0.43 2016 Equine 2016 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Cantarero, Irene (orcid)0000-0002-4495-8857 Ranera, Beatriz (orcid)0000-0002-1075-8267 Remacha, Ana Rosa Universidad de Zaragoza (orcid)0000-0001-7453-2470 Castiella, Tomás Universidad de Zaragoza Romero, Antonio (orcid)0000-0001-6016-4726 Martín Inmaculada Universidad de Zaragoza (orcid)0000-0003-3289-2675 Rodellar, Clementina Universidad de Zaragoza (orcid)0000-0002-9951-1075 Junquera, Concepción Universidad de Zaragoza 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1003 027 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Anatom.Embriol.Humana 1000 020 Universidad de Zaragoza Dpto. Anat.Pat.Med.Leg.For.To. Area Anatomía Patológica 47 (2016), 47-54 J. equine vet. sci. JOURNAL OF EQUINE VETERINARY SCIENCE 0737-0806 2055251 http://zaguan.unizar.es/record/57068/files/texto_completo.pdf Postprint 44032 http://zaguan.unizar.es/record/57068/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:57068 articulos driver 2020-08-13-07:57:32 ARTICLE