000057086 001__ 57086 000057086 005__ 20200221144220.0 000057086 0247_ $$2doi$$a10.1371/journal.pone.0163729 000057086 0248_ $$2sideral$$a96710 000057086 037__ $$aART-2016-96710 000057086 041__ $$aeng 000057086 100__ $$aLópez-Soriano, P. 000057086 245__ $$aMultilocus variable number of tandem repeat analysis reveals multiple introductions in Spain of Xanthomonas arboricola pv. Pruni, the causal agent of bacterial spot disease of stone fruits and almond 000057086 260__ $$c2016 000057086 5060_ $$aAccess copy available to the general public$$fUnrestricted 000057086 5203_ $$aXanthomonas arboricola pv. pruni is the causal agent of the bacterial spot disease of stone fruits, almond and some ornamental Prunus species. In Spain it was first detected in 2002 and since then, several outbreaks have occurred in different regions affecting mainly Japanese plum, peach and almond, both in commercial orchards and nurseries. As the origin of the introduction(s) was unknown, we have assessed the genetic diversity of 239 X. arboricola pv. pruni strains collected from 11 Spanish provinces from 2002 to 2013 and 25 reference strains from international collections. We have developed an optimized multilocus variable number of tandem repeat analysis (MLVA) scheme targeting 18 microsatellites and five minisatellites. A high discriminatory power was achieved since almost 50% of the Spanish strains were distinguishable, confirming the usefulness of this genotyping technique at small spatio-temporal scales. Spanish strains grouped in 18 genetic clusters (conservatively delineated so that each cluster contained haplotype networks linked by up to quadruple-locus variations). Furthermore, pairwise comparisons among populations from different provinces showed a strong genetic differentiation. Our results suggest multiple introductions of this pathogen in Spain and redistribution through contaminated nursery propagative plant material. 000057086 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2011-00140-C03-01$$9info:eu-repo/grantAgreement/EC/FP7/266505/EU/EUROPEAN PHYTOSANITARY RESEARCH COORDINATION II/EUPHRESCO 000057086 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000057086 590__ $$a2.806$$b2016 000057086 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1 000057086 592__ $$a1.236$$b2016 000057086 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1 000057086 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1 000057086 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1 000057086 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000057086 700__ $$aBoyer, K. 000057086 700__ $$aCesbron, S. 000057086 700__ $$aMorente, M.C. 000057086 700__ $$aPeñalver, J. 000057086 700__ $$0(orcid)0000-0002-3777-8612$$aPalacio-Bielsa, A. 000057086 700__ $$aVernière, C. 000057086 700__ $$aLópez, M.M. 000057086 700__ $$aPruvost, O. 000057086 773__ $$g11, 9 (2016), 0163729[18pp]$$pPLoS One$$tPloS one$$x1932-6203 000057086 8564_ $$s2127355$$uhttps://zaguan.unizar.es/record/57086/files/texto_completo.pdf$$yVersión publicada 000057086 8564_ $$s102979$$uhttps://zaguan.unizar.es/record/57086/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000057086 909CO $$ooai:zaguan.unizar.es:57086$$particulos$$pdriver 000057086 951__ $$a2020-02-21-13:14:58 000057086 980__ $$aARTICLE