000057093 001__ 57093
000057093 005__ 20200909105805.0
000057093 0247_ $$2doi$$a10.1371/journal.pone.0163770
000057093 0248_ $$2sideral$$a96704
000057093 037__ $$aART-2016-96704
000057093 041__ $$aeng
000057093 100__ $$aHurtado-Roca, Y.
000057093 245__ $$aAdjusting MtDNA quantification in whole blood for peripheral blood platelet and leukocyte counts
000057093 260__ $$c2016
000057093 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057093 5203_ $$aAlterations of mitochondrial DNA copy number (mtDNAcn) in the blood (mitochondrial to nuclear DNA ratio) appear associated with several systemic diseases, including primary mitochondrial disorders, carcinogenesis, and hematologic diseases. Measuring mtDNAcn in DNA extracted from whole blood (WB) instead of from peripheral blood mononuclear cells or buffy coat may yield different results due to mitochondrial DNA present in platelets. The aim of this work is to quantify the contribution of platelets to mtDNAcn in whole blood mtDNAcn(WB)] and to propose a correction formula to estimate leukocytes'' mtDNAcn mtDNAcn(L)] from mtDNAcn(WB). Blood samples from 10 healthy adults were combined with platelet-enriched plasma and saline solution to produce artificial blood preparations. Aliquots of each sample were combined with five different platelet concentrations. In 46 of these blood preparations, mtDNAcn was measured by qPCR. MtDNAcn(WB) increased 1.07 (95%CI 0.86, 1.29; p<0.001) per 1000 platelets present in the preparation. We proved that leukocyte count should also be taken into account as mtDNAcn(WB) was inversely associated with leukocyte count; it increased 1.10 (95%CI 0.95, 1.25, p<0.001) per unit increase of the ratio between platelet and leukocyte counts. If hematological measurements are available, subtracting 1.10 the platelets/leukocyte ratio from mtDNAcn(WB) may serve as an estimation for mtDNAcn(L). Both platelet and leukocyte counts in the sample are important sources of variation if comparing mtDNAcn among groups of patients when mtDNAcn is measured in DNA extracted from whole blood. Not taking the platelet/leukocyte ratio into account in whole blood measurements, may lead to overestimation and misclassification if interpreted as leukocytes'' mtDNAcn.
000057093 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI14-00009$$9info:eu-repo/grantAgreement/ES/FIS/PI10-00021
000057093 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057093 590__ $$a2.806$$b2016
000057093 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000057093 592__ $$a1.236$$b2016
000057093 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000057093 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000057093 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000057093 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057093 700__ $$aLedesma, M.
000057093 700__ $$aGonzalez-Lazaro, M.
000057093 700__ $$0(orcid)0000-0002-6600-1618$$aMoreno-Loshuertos, R.$$uUniversidad de Zaragoza
000057093 700__ $$0(orcid)0000-0001-8971-7355$$aFernandez-Silva, P.$$uUniversidad de Zaragoza
000057093 700__ $$aEnriquez, J.A.
000057093 700__ $$aLaclaustra, M.
000057093 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000057093 773__ $$g11, 10 (2016), 0163770 [14 pp]$$pPLoS One$$tPloS one$$x1932-6203
000057093 8564_ $$s1911812$$uhttps://zaguan.unizar.es/record/57093/files/texto_completo.pdf$$yVersión publicada
000057093 8564_ $$s104421$$uhttps://zaguan.unizar.es/record/57093/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057093 909CO $$ooai:zaguan.unizar.es:57093$$particulos$$pdriver
000057093 951__ $$a2020-09-09-10:49:21
000057093 980__ $$aARTICLE