000057769 001__ 57769
000057769 005__ 20200221144225.0
000057769 0247_ $$2doi$$a10.4103/1008-682X.186877
000057769 0248_ $$2sideral$$a97133
000057769 037__ $$aART-2016-97133
000057769 041__ $$aeng
000057769 100__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.$$uUniversidad de Zaragoza
000057769 245__ $$aA comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
000057769 260__ $$c2016
000057769 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057769 5203_ $$aThe aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle (Bos taurus), sheep (Ovis aries), and pigs (Sus scrofa). Semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum agglutinin (PI/PSA) combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed. The use of the PI/PSA combination and CASA-Morph fluorescence-based method allowed the capture, morphometric analysis, and differentiation of most sperm nuclei, acrosomes and whole heads, and the assessment of acrosomal integrity with a high precision in the three species studied. For the size of the head and nuclear area, the relationship between the three species may be summarized as bull > ram > boar. However, for the other morphometric parameters (length, width, and perimeter), there were differences in the relationships between species for sperm nuclei and whole sperm heads. Bull sperm acrosomes were clearly smaller than those in the other species studied and covered a smaller proportion of the sperm head. The acrosomal morphology, small in the bull, large and broad in the sheep, and large, long, and with a pronounced equatorial segment curve in the boar, was species-characteristic. It was concluded that there are clear variations in the size and shape of the sperm head components between the three species studied, the acrosome being the structure showing the most variability, allowing a clear distinction of the spermatozoa of each species.
000057769 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A40$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P
000057769 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057769 590__ $$a2.996$$b2016
000057769 591__ $$aANDROLOGY$$b1 / 5 = 0.2$$c2016$$dQ1$$eT1
000057769 591__ $$aUROLOGY & NEPHROLOGY$$b22 / 76 = 0.289$$c2016$$dQ2$$eT1
000057769 592__ $$a0.793$$b2016
000057769 593__ $$aUrology$$c2016$$dQ2
000057769 593__ $$aMedicine (miscellaneous)$$c2016$$dQ2
000057769 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057769 700__ $$aCapistrós, S.
000057769 700__ $$aVicente-Fiel, S.
000057769 700__ $$aHidalgo, C.
000057769 700__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, P.$$uUniversidad de Zaragoza
000057769 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000057769 773__ $$g18, 6 (2016), 840-843$$pAsian J. Androl.$$tAsian journal of andrology$$x1008-682X
000057769 8564_ $$s407082$$uhttps://zaguan.unizar.es/record/57769/files/texto_completo.pdf$$yVersión publicada
000057769 8564_ $$s129608$$uhttps://zaguan.unizar.es/record/57769/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057769 909CO $$ooai:zaguan.unizar.es:57769$$particulos$$pdriver
000057769 951__ $$a2020-02-21-13:17:27
000057769 980__ $$aARTICLE