000057770 001__ 57770
000057770 005__ 20200221144225.0
000057770 0247_ $$2doi$$a10.4103/1008-682X.186872
000057770 0248_ $$2sideral$$a97134
000057770 037__ $$aART-2016-97134
000057770 041__ $$aeng
000057770 100__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.$$uUniversidad de Zaragoza
000057770 245__ $$aComparison of different statistical approaches to evaluate morphometric sperm subpopulations in men
000057770 260__ $$c2016
000057770 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057770 5203_ $$aThis study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphometric determination. At least 200 spermatozoa per sample were assessed for sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence. Clustering and discriminant procedures were performed to identify sperm subpopulations from the morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three morphometric subpopulations (large-round 30.4%, small-round 46.6%, and large-elongated 22.9%). In the second analysis, using discriminant methods, the classification was made independently of size and shape. Three morphological categories according to nuclear size (small 13.07 µm2) and four categories were defined on 400 canonical cells (100 × 4) from 10 men according to sperm nuclear shape (oval, pyriform, round, and elongated). Thereafter, the resulting classification functions were used to categorize 4200 spermatozoa from 21 men. Differences in the class distribution were observed among men from both clustering and discriminant procedures. It was concluded that the combination of CASA-Morph fluorescence-based technology with multivariate cluster or discriminant analyses provides new information on the description of different morphometric sperm subpopulations in normal individuals, and that important variations in the distribution of morphometric sperm subpopulations may exist between men, with possible functional implications.
000057770 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A40$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P
000057770 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057770 590__ $$a2.996$$b2016
000057770 591__ $$aANDROLOGY$$b1 / 5 = 0.2$$c2016$$dQ1$$eT1
000057770 591__ $$aUROLOGY & NEPHROLOGY$$b22 / 76 = 0.289$$c2016$$dQ2$$eT1
000057770 592__ $$a0.793$$b2016
000057770 593__ $$aUrology$$c2016$$dQ2
000057770 593__ $$aMedicine (miscellaneous)$$c2016$$dQ2
000057770 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057770 700__ $$aVicente-Fiel, S.
000057770 700__ $$aSoler, C.
000057770 700__ $$0(orcid)0000-0001-5359-6037$$aRecreo, P.$$uUniversidad de Zaragoza
000057770 700__ $$0(orcid)0000-0001-6202-3089$$aCarretero, T.$$uUniversidad de Zaragoza
000057770 700__ $$aBono, A.
000057770 700__ $$aBerné, J.
000057770 700__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, P.$$uUniversidad de Zaragoza
000057770 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000057770 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000057770 773__ $$g18, 6 (2016), 819-823$$pAsian J. Androl.$$tAsian journal of andrology$$x1008-682X
000057770 8564_ $$s438488$$uhttps://zaguan.unizar.es/record/57770/files/texto_completo.pdf$$yVersión publicada
000057770 8564_ $$s128250$$uhttps://zaguan.unizar.es/record/57770/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057770 909CO $$ooai:zaguan.unizar.es:57770$$particulos$$pdriver
000057770 951__ $$a2020-02-21-13:17:31
000057770 980__ $$aARTICLE