000057789 001__ 57789
000057789 005__ 20200221144319.0
000057789 0247_ $$2doi$$a10.1002/anie.201608438
000057789 0248_ $$2sideral$$a97077
000057789 037__ $$aART-2016-97077
000057789 041__ $$aeng
000057789 100__ $$aOliveira, B.L.
000057789 245__ $$aA Minimal, Unstrained S-Allyl Handle for Pre-Targeting Diels–Alder Bioorthogonal Labeling in Live Cells
000057789 260__ $$c2016
000057789 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057789 5203_ $$aThe unstrained S-allyl cysteine amino acid was site-specifically installed on apoptosis protein biomarkers and was further used as a chemical handle and ligation partner for 1, 2, 4, 5-tetrazines by means of an inverse-electron-demand Diels–Alder reaction. We demonstrate the utility of this minimal handle for the efficient labeling of apoptotic cells using a fluorogenic tetrazine dye in a pre-targeting approach. The small size, easy chemical installation, and selective reactivity of the S-allyl handle towards tetrazines should be readily extendable to other proteins and biomolecules, which could facilitate their labeling within live cells.
000057789 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-70524-R$$9info:eu-repo/grantAgreement/ES/MICINN/RYC-2013-14706
000057789 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057789 590__ $$a11.994$$b2016
000057789 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b13 / 166 = 0.078$$c2016$$dQ1$$eT1
000057789 592__ $$a5.953$$b2016
000057789 593__ $$aChemistry (miscellaneous)$$c2016$$dQ1
000057789 593__ $$aCatalysis$$c2016$$dQ1
000057789 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057789 700__ $$aGuo, Z.
000057789 700__ $$aBoutureira, O.
000057789 700__ $$aGuerreiro, A.
000057789 700__ $$0(orcid)0000-0003-0105-4337$$aJiménez-Osés, G.
000057789 700__ $$aBernardes, G.J.L.
000057789 773__ $$g55, 47 (2016), 14683-14687$$pAngew. Chem. (Int. ed.)$$tAngewandte Chemie (International ed.)$$x1433-7851
000057789 8564_ $$s2025815$$uhttps://zaguan.unizar.es/record/57789/files/texto_completo.pdf$$yVersión publicada
000057789 8564_ $$s129205$$uhttps://zaguan.unizar.es/record/57789/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057789 909CO $$ooai:zaguan.unizar.es:57789$$particulos$$pdriver
000057789 951__ $$a2020-02-21-13:40:48
000057789 980__ $$aARTICLE