Morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men

Santolaria, P. (Universidad de Zaragoza) ; Soler, C. ; Recreo, P. (Universidad de Zaragoza) ; Carretero, T. (Universidad de Zaragoza) ; Bono, A. ; Berné, J. ; Yániz, J. (Universidad de Zaragoza)

Resumen: This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations (slow with low ALH 35.6% of all motile spermatozoa], with circular trajectories 32.0%], and rapid with high ALH 32.4%]), and three morphometric subpopulations (large-round 33.9% of all spermatozoa], elongated 32.0%], and small 34.10%]). The distribution of kinematic sperm subpopulations was different among ejaculate fractions (P < 0.001), with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions (P < 0.001), with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.

Idioma: Inglés

DOI: 10.4103/1008-682X.186874

Año: 2016

Publicado en: Asian journal of andrology 18, 6 (2016), 831-834
ISSN: 1008-682X

Factor impacto JCR: 2.996 (2016)

Categ. JCR: ANDROLOGY rank: 1 / 5 = 0.2 (2016) - Q1 - T1
Categ. JCR: UROLOGY & NEPHROLOGY rank: 22 / 76 = 0.289 (2016) - Q2 - T1

Factor impacto SCIMAGO: 0.793 - Urology (Q2) - Medicine (miscellaneous) (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A40
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Area Anatom.Embriol.Humana (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)