57798 20200221144337.0 doi 10.4103/1008-682X.186874 sideral 97112 ART-2016-97112 eng (orcid)0000-0001-8991-325X Santolaria, P. Universidad de Zaragoza Morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men 2016 Access copy available to the general public Unrestricted This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations (slow with low ALH 35.6% of all motile spermatozoa], with circular trajectories 32.0%], and rapid with high ALH 32.4%]), and three morphometric subpopulations (large-round 33.9% of all spermatozoa], elongated 32.0%], and small 34.10%]). The distribution of kinematic sperm subpopulations was different among ejaculate fractions (P < 0.001), with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions (P < 0.001), with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications. info:eu-repo/grantAgreement/ES/DGA/A40 info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.996 2016 ANDROLOGY 1 / 5 = 0.2 2016 Q1 T1 UROLOGY & NEPHROLOGY 22 / 76 = 0.289 2016 Q2 T1 0.793 2016 Urology 2016 Q2 Medicine (miscellaneous) 2016 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Soler, C. (orcid)0000-0001-5359-6037 Recreo, P. Universidad de Zaragoza (orcid)0000-0001-6202-3089 Carretero, T. Universidad de Zaragoza Bono, A. Berné, J. (orcid)0000-0001-5316-1703 Yániz, J. Universidad de Zaragoza 1003 027 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Anatom.Embriol.Humana 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 18, 6 (2016), 831-834 Asian J. Androl. Asian journal of andrology 1008-682X 252340 http://zaguan.unizar.es/record/57798/files/texto_completo.pdf Versión publicada 128653 http://zaguan.unizar.es/record/57798/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:57798 articulos driver 2020-02-21-13:48:17 ARTICLE