57908 20170327111930.0 doi 10.1155/2014/287896 sideral 88580 ART-2014-88580 eng Srivastava, Girish Kumar Chitosan feasibility to retain retinal stem cell phenotype and slow proliferation for retinal transplantation 2014 Access copy available to the general public Unrestricted Retinal stem cells (RSCs) are promising in cell replacement strategies for retinal diseases. RSCs can migrate, differentiate, and integrate into retina. However, RSCs transplantation needs an adequate support; chitosan membrane (ChM) could be one, which can carry RSCs with high feasibility to support their integration into retina. RSCs were isolated, evaluated for phenotype, and subsequently grown on sterilized ChM and polystyrene surface for 8 hours, 1, 4, and 11 days for analysing cell adhesion, proliferation, viability, and phenotype. Isolated RSCs expressed GFAP, PKC, isolectin, recoverin, RPE65, PAX-6, cytokeratin 8/18, and nestin proteins. They adhered (28 ± 16%, 8 hours) and proliferated (40 ± 20 cells/field, day 1 and 244 ± 100 cells/field, day 4) significantly low on ChM. However, they maintained similar viability (>95%) and phenotype (cytokeratin 8/18, PAX6, and nestin proteins expression, day 11) on both surfaces (ChM and polystyrene). RSCs did not express alpha-SMA protein on both surfaces. RSCs express proteins belonging to epithelial, glial, and neural cells, confirming that they need further stimulus to reach a final destination of differentiation that could be provided in in vivo condition. ChM does not alternate RSCs behaviour and therefore can be used as a cell carrier so that slow proliferating RSCs can migrate and integrate into retina. info:eu-repo/grantAgreement/ES/MICINN/ISCIII-PS09-00938 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 1.579 2014 MEDICINE, RESEARCH & EXPERIMENTAL 85 / 123 = 0.691 2014 Q3 T3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 106 / 162 = 0.654 2014 Q3 T2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Rodríguez-Crespo, David Singh, Amar Kumar Casado-Coterillo, Clara Fernandez-Bueno, Ivan Garcia-Gutierrez, Maria T. (orcid)0000-0003-1512-4500 Coronas, Joaquin Universidad de Zaragoza Pastor, J. Carlos 5005 555 Universidad de Zaragoza Departamento de Ingeniería Química y Tecnologías del Medio Ambiente Ingeniería Química 2014 (2014), 287896 [10 pp] BioMed res. int. BioMed Research International 2314-6133 3625269 http://zaguan.unizar.es/record/57908/files/texto_completo.pdf Versión publicada 101680 http://zaguan.unizar.es/record/57908/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:57908 articulos driver 2016-12-16-14:53:32 ARTICLE