000057909 001__ 57909
000057909 005__ 20170327111931.0
000057909 0247_ $$2doi$$a10.1155/2013/513932
000057909 0248_ $$2sideral$$a81388
000057909 037__ $$aART-2013-81388
000057909 041__ $$aeng
000057909 100__ $$aLanaspa, M.A.
000057909 245__ $$aInorganic phosphate modulates the expression of the NaPi-2a transporter in the trans -golgi network and the interaction with PIST in the proximal tubule
000057909 260__ $$c2013
000057909 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057909 5203_ $$aInorganic phosphate (Pi) homeostasis is maintained by the tight regulation of renal Pi excretion versus reabsorption rates that are in turn modulated by adjusting the number of Pi transporters (mainly NaPi-2a) in the proximal tubules. In response to some hormones and a high dietary Pi content, NaPi-2a is endocytosed and degraded in the lysosomes; however, we show here that some NaPi-2amolecules are targeted to the trans-Golgi network (TGN) during the endocytosis. In the TGN, NaPi-2a interacts with PIST (PDZ-domain protein interacting specifically with TC10), a TGN-resident PDZ-domain-containing protein. The extension of the interaction is proportional to the expression of NaPi-2a in the TGN, and, consistent with that, it is increased with a high Pi diet. When overexpressed in opossum kidney (OK) cells, PIST retains NaPi-2a in the TGN and inhibits Na-dependent Pi transport. Overexpression of PIST also prevents the adaptation of OK cells to a low Pi culture medium. Our data supports the view that NaPi-2a is subjected to retrograde trafficking from the plasma membrane to the TGN using one of the machineries involved in endosomal transport and explains the reported expression of NaPi-2a in the TGN.
000057909 536__ $$9info:eu-repo/grantAgreement/ES/DGA/PI020-09$$9info:eu-repo/grantAgreement/ES/MICINN/BFU2009-12763-BFI
000057909 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057909 590__ $$a0.0$$b2013
000057909 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057909 700__ $$aCaldas, Y.A.
000057909 700__ $$aBreusegem, S.Y.
000057909 700__ $$aAndrés-Hernando, A.
000057909 700__ $$aCicerchi, C.
000057909 700__ $$aLevi, M.
000057909 700__ $$0(orcid)0000-0003-3457-323X$$aSorribas, V.$$uUniversidad de Zaragoza
000057909 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDepartamento de Anatomía Patológica, Medicina Legal y Forense y Toxicología$$cToxicología
000057909 773__ $$g2013 (2013), 513932 [9 pp]$$pBioMed res. int.$$tBioMed Research International$$x2314-6133
000057909 8564_ $$s9054491$$uhttps://zaguan.unizar.es/record/57909/files/texto_completo.pdf$$yVersión publicada
000057909 8564_ $$s98880$$uhttps://zaguan.unizar.es/record/57909/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057909 909CO $$ooai:zaguan.unizar.es:57909$$particulos$$pdriver
000057909 951__ $$a2016-12-16-14:53:35
000057909 980__ $$aARTICLE