58389 20210121114532.0 doi 10.1167/iovs.15-16808 sideral 92002 ART-2015-92002 eng Garcia-Ayuso D Inherited photoreceptor degeneration causes the death of melanopsin-positive retinal ganglion cells and increases their coexpression of brn3a 2015 Access copy available to the general public Unrestricted Purpose: To study the population of intrinsically photosensitive retinal ganglion cells (melanopsin-expressing RGCs, m+RGCs) in P23H-1 rats, a rat model of inherited photoreceptor degeneration. Methods: At postnatal (P) times P30, P365, and P540, retinas from P23H dystrophic rats (line 1, rapid degeneration; and line 3, slow degeneration) and Sprague Dawley (SD) rats (control) were dissected as whole-mounts and immunodetected for melanopsin and/or Brn3a. The dendritic arborization of m+RGCs and the numbers of Brn3a+RGCs and m+RGCs were quantified and their retinal distribution and coexpression analyzed. Results: In SD rats, aging did not affect the population of Brn3a+RGCs or m+RGCs or the percentage that showed coexpression (0.27%). Young P23H-1 rats had a significantly lower number of Brn3a+RGCs and showed a further decline with age. The population of m+RGCs in young P23H-1 rats was similar to that found in SD rats and decreased by 22.6% and 28.2% at P365 and P540, respectively, similarly to the decrease of the Brn3a+RGCs. At these ages the m+RGCs showed a decrease of their dendritic arborization parameters, which was similar in both the P23H-1 and P23H-3 lines. The percentage of coexpression of Brn3a was, however, already significantly higher at P30 (3.31%) and increased significantly with age (10.65% at P540). Conclusions: Inherited photoreceptor degeneration was followed by secondary loss of Brn3a+RGCs and m+RGCs. Surviving m+RGCs showed decreased dendritic arborization parameters and increased coexpression of Brn3a and melanopsin, phenotypic and molecular changes that may represent an effort to resist degeneration and/or preferential survival of m+RGCs capable of synthesizing Brn3a. info:eu-repo/grantAgreement/ES/ISCIII/PI13-00643 info:eu-repo/grantAgreement/ES/ISCIII/PI13-01266 info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD12-0034-0010 info:eu-repo/grantAgreement/ES/MICINN/ISCIII-RD12-0034-0014 info:eu-repo/grantAgreement/ES/MINECO/BFU2012-36845 info:eu-repo/grantAgreement/ES/MINECO/SAF-2012-38328 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.427 2015 OPHTHALMOLOGY 6 / 56 = 0.107 2015 Q1 T1 2.011 2015 Ophthalmology 2015 Q1 Sensory Systems 2015 Q1 Cellular and Molecular Neuroscience 2015 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Di Pierdomenico J Esquiva G Nadal-Nicolás FM (orcid)0000-0003-0349-9997 Pinilla I Universidad de Zaragoza Cuenca N Vidal-Sanz M Agudo-Barriuso M Villegas-Pérez MP. 1004 646 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Oftalmología 56, 8 (2015), 4592-4604 Investig. ophthalmol. vis. sci. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 0146-0404 2037843 http://zaguan.unizar.es/record/58389/files/texto_completo.pdf Versión publicada 127462 http://zaguan.unizar.es/record/58389/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:58389 articulos driver 2021-01-21-11:11:06 ARTICLE