000058480 001__ 58480
000058480 005__ 20200221144314.0
000058480 0247_ $$2doi$$a10.1371/journal.pone.0167171
000058480 0248_ $$2sideral$$a97595
000058480 037__ $$aART-2016-97595
000058480 041__ $$aeng
000058480 100__ $$aThi, V.H.D.
000058480 245__ $$aRecreating stable Brachypodium hybridum allotetraploids by uniting the divergent genomes of B. distachyon and B. stacei
000058480 260__ $$c2016
000058480 5060_ $$aAccess copy available to the general public$$fUnrestricted
000058480 5203_ $$aBrachypodium hybridum (2n = 30) is a natural allopolyploid with highly divergent subgenomes derived from two extant diploid species, B. distachyon (2n = 10) and B. stacei (2n = 20) that differ in chromosome evolution and number. We created synthetic B. hybridum allotetraploids by hybridizing various lines of B. distachyon and B. stacei. The initial amphihaploid F1 interspecific hybrids were obtained at low frequencies when B. distachyon was used as the maternal parent (0.15% or 0.245% depending on the line used) and were sterile. No hybrids were obtained from reciprocal crosses or when autotetraploids of the parental species were crossed. Colchicine treatment was used to double the genome of the F1 amphihaploid lines leading to allotetraploids. The genome-doubled F1 plants produced a few S1 (first selfed generation) seeds after self-pollination. S1 plants from one parental combination (Bd3-1×Bsta5) were fertile and gave rise to further generations whereas those of another parental combination (Bd21×ABR114) were sterile, illustrating the importance of the parental lineages crossed. The synthetic allotetraploids were stable and resembled the natural B. hybridum at the phenotypic, cytogenetic and genomic levels. The successful creation of synthetic B. hybridum offers the possibility to study changes in genome structure and regulation at the earliest stages of allopolyploid formation in comparison with the parental species and natural B. hybridum. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
000058480 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000058480 590__ $$a2.806$$b2016
000058480 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000058480 592__ $$a1.236$$b2016
000058480 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000058480 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000058480 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000058480 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000058480 700__ $$aCoriton, O.
000058480 700__ $$aLe Clainche, I.
000058480 700__ $$aArnaud, D.
000058480 700__ $$aGordon, S.P.
000058480 700__ $$aLinc, G.
000058480 700__ $$0(orcid)0000-0001-7793-5259$$aCatalan, P.$$uUniversidad de Zaragoza
000058480 700__ $$aHasterok, R.
000058480 700__ $$aVogel, J.P.
000058480 700__ $$aJahier, J.
000058480 700__ $$aChalhoub, B.
000058480 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000058480 773__ $$g11, 12 (2016), 0167171[27 pp]$$pPLoS One$$tPloS one$$x1932-6203
000058480 8564_ $$s1009862$$uhttps://zaguan.unizar.es/record/58480/files/texto_completo.pdf$$yVersión publicada
000058480 8564_ $$s105923$$uhttps://zaguan.unizar.es/record/58480/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000058480 909CO $$ooai:zaguan.unizar.es:58480$$particulos$$pdriver
000058480 951__ $$a2020-02-21-13:39:06
000058480 980__ $$aARTICLE