000058535 001__ 58535
000058535 005__ 20190513085213.0
000058535 0247_ $$2doi$$a10.1186/1297-9716-44-14
000058535 0248_ $$2sideral$$a81318
000058535 037__ $$aART-2013-81318
000058535 041__ $$aeng
000058535 100__ $$0(orcid)0000-0003-3352-740X$$aFilali, H.$$uUniversidad de Zaragoza
000058535 245__ $$aGene and protein patterns of potential prion-related markers in the central nervous system of clinical and preclinical infected sheep
000058535 260__ $$c2013
000058535 5060_ $$aAccess copy available to the general public$$fUnrestricted
000058535 5203_ $$aThe molecular pathogenic mechanisms of prion diseases are far from clear. Genomic analyses have revealed genetic biomarkers potentially involved in prion neuropathology in naturally scrapie-infected sheep, a good animal model of infectious prionopathies. However, these biomarkers must be validated in independent studies at different stages of the disease. The gene and protein expression profiles and protein distribution of six potential genetic biomarkers (i.e., CAPN6, COL1A2, COL3A1, GALA1, MT2A and MTNR1B) are presented here for both the early and terminal stages of scrapie in five different brain regions. Gene transcription changes were confirmed in the medulla oblongata, and the expression profiles were generally similar in other central nervous system regions. The changes were more substantial in clinical animals compared to preclinical animals. The expression of the CAPN6 protein increased in the spinal cord and cerebellum of the clinical and preclinical brains. The distribution of the GALA1 was identified in glial cells from the cerebellum of scrapie-infected animals, GALA1 protein expression was increased in clinical animals in the majority of regions, and the increase of MT2A was in agreement with previous reports. The downregulation of MTNR1B was especially marked in the Purkinje cells. Finally, although collagen genes were downregulated the protein immunostaining did not reveal significant changes between the scrapie-infected and control animals. In conclusion, this study of gene transcription and protein expression and distribution confirm CAPN6, GALA1, MTNR1B and MT2A as potential targets for further prion disease research.
000058535 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2008-0256
000058535 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000058535 590__ $$a3.383$$b2013
000058535 591__ $$aVETERINARY SCIENCES$$b1 / 132 = 0.008$$c2013$$dQ1$$eT1
000058535 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000058535 700__ $$aVidal, E.
000058535 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, R.$$uUniversidad de Zaragoza
000058535 700__ $$aMárquez, M.
000058535 700__ $$aMarco, P.
000058535 700__ $$0(orcid)0000-0002-6158-691X$$aVargas, A.$$uUniversidad de Zaragoza
000058535 700__ $$0(orcid)0000-0002-0935-7941$$aPumarola, M.
000058535 700__ $$0(orcid)0000-0001-6016-4726$$aMartin-Burriel, I.$$uUniversidad de Zaragoza
000058535 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza
000058535 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000058535 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000058535 773__ $$g44, 14 (2013), [17 pp]$$pVet. res.$$tVeterinary Research$$x0928-4249
000058535 8564_ $$s1668435$$uhttps://zaguan.unizar.es/record/58535/files/texto_completo.pdf$$yVersión publicada
000058535 8564_ $$s110949$$uhttps://zaguan.unizar.es/record/58535/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000058535 909CO $$ooai:zaguan.unizar.es:58535$$particulos$$pdriver
000058535 951__ $$a2019-05-13-08:49:21
000058535 980__ $$aARTICLE