doi:10.1016/j.diagmicrobio.2016.02.021engGonzález-Domínguez, MariaSeral, CristinaPotel, CarmenSáenz, YolandaAlvarez, MaximilianoTorres, CarmenCastillo, Francisco JavierGenotypic and phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clones with high-level mupirocin resistanceART-2016-94412An elevated proportion (27.2%) of high-level mupirocin-resistant methicillin-resistant Staphylococcus aureus (HLMUPR-MRSA) isolates were found in our environment in one year period. HLMUPR-MRSA isolates were mainly collected from skin and soft tissue samples, and diabetes was the main related comorbidity condition. These isolates were more frequently found in vascular surgery. HLMUPR-MRSA were more resistant to aminoglycosides than mupirocin-susceptible MRSA, linked to the presence of bifunctional and/or nucleotidyltransferase enzymes with/without macrolide resistance associated with the msr(A) gene. Most of HLMUPR-MRSA isolates belonged to ST125/t067. Nine IS257-ileS2 amplification patterns (p3 was the most frequent) were observed in HLMUPR-MRSA isolates, suggesting the presence of several mupirocin-resistance-carrying plasmids in our environment and promoting the emergence of mupirocin resistance. The presence of the same IS257-ileS2 amplification pattern p3 in 65% of HLMUPR-MRSA, all of them ST125/t067, suggests a clonal spread in our hospital and community environment which could explain the high prevalence of HLMUPR-MRSA during the study period. An outbreak situation or an increase in mupirocin consumption was not observed.2016http://zaguan.unizar.es/record/6060610.1016/j.diagmicrobio.2016.02.021http://zaguan.unizar.es/record/60606oai:zaguan.unizar.es:60606info:eu-repo/grantAgreement/ES/DGA/B24-211130Diagnostic Microbiology and Infectious Disease 85 (2016), 213-217by-nc-ndhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccess