60656 20200217133050.0 doi 10.3390/toxins6010394 sideral 98047 ART-2014-98047 eng Giménez, Isabel Effects of Bread Making and Wheat Germ Addition on the Natural Deoxynivalenol Content in Bread 2014 Access copy available to the general public Unrestricted Deoxynivalenol (DON, vomitoxin) is a type-B trichothecene mycotoxin produced by several field fungi such as Fusarium graminearum and Fusarium culmorum and known to have various toxic effects. This study investigated the effect of the bread making process on the stability of DON in common bread and wheat germ-enriched bread using naturally contaminated ingredients at the level of 560 µg/kg. The concentration of DON and its evolution during bread making were determined by immunoaffinity column cleanup followed by liquid chromatography with diode array detection (HPLC-DAD). During the bread making process, DON was reduced by 2.1% after fermentation and dropped by 7.1% after baking, reaching a maximum reduction of 19.8% in the crust as compared with a decrease of 5.6% in the crumb. The addition of 15% wheat germ to the dough did not affect DON stability during bread making, showing an apparent increase of 3.5% after fermentation and a reduction by 10.2% after baking. info:eu-repo/grantAgreement/ES/DGA/A01 info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2011-26808 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.938 2014 TOXICOLOGY 28 / 88 = 0.318 2014 Q2 T1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Blesa, Jesús (orcid)0000-0002-2469-0363 Herrera, Marta Universidad de Zaragoza (orcid)0000-0001-6325-7100 Ariño, Agustín Universidad de Zaragoza 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 6, 1 (2014), 394-401 Toxins Toxins (Basel) 2072-6651 359749 http://zaguan.unizar.es/record/60656/files/texto_completo.pdf Versión publicada 83243 http://zaguan.unizar.es/record/60656/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:60656 articulos driver 2020-02-17-12:42:25 ARTICLE