000060668 001__ 60668
000060668 005__ 20190709135436.0
000060668 0247_ $$2doi$$a10.1371/journal.pone.0171417
000060668 0248_ $$2sideral$$a98076
000060668 037__ $$aART-2017-98076
000060668 041__ $$aeng
000060668 100__ $$aAnguiano, M.
000060668 245__ $$aCharacterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis
000060668 260__ $$c2017
000060668 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060668 5203_ $$aMicrofluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues. In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments. We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions. Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue. Our quantification of migration speed confirms a biphasic role of Matrigel. At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment. At high concentration, Matrigel slows down migration, possibly due excessive attachment. Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix.In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions. This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
000060668 536__ $$9info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751$$9info:eu-repo/grantAgreement/ES/ISCIII/PI13-02313$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2012-38090-C03-02$$9info:eu-repo/grantAgreement/ES/MINECO/PTQ-11-04778$$9info:eu-repo/grantAgreement/ES/MINECO/TEC2013-48552-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/TEC2015-73064-EXP$$9info:eu-repo/grantAgreement/ES/MINECO/TEC2016-78052-R
000060668 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000060668 590__ $$a2.766$$b2017
000060668 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 64 = 0.234$$c2017$$dQ1$$eT1
000060668 592__ $$a1.164$$b2017
000060668 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2017$$dQ1
000060668 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000060668 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2017$$dQ1
000060668 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060668 700__ $$aCastilla, C.
000060668 700__ $$aMaška, M.
000060668 700__ $$aEderra, C.
000060668 700__ $$aPeláez, R.
000060668 700__ $$aMorales, X.
000060668 700__ $$aMuñoz-Arrieta, G.
000060668 700__ $$aMujika, M.
000060668 700__ $$aKozubek, M.
000060668 700__ $$aMuñoz-Barrutia, A.
000060668 700__ $$aRouzaut, A.
000060668 700__ $$aArana, S.
000060668 700__ $$0(orcid)0000-0002-9864-7683$$aGarcia-Aznar, J.$$uUniversidad de Zaragoza
000060668 700__ $$aOrtiz-de-Solorzano, C.
000060668 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000060668 773__ $$g12, 2 (2017), 0171417 [24 p.]$$pPLoS One$$tPloS one$$x1932-6203
000060668 8564_ $$s3810486$$uhttps://zaguan.unizar.es/record/60668/files/texto_completo.pdf$$yVersión publicada
000060668 8564_ $$s106467$$uhttps://zaguan.unizar.es/record/60668/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000060668 909CO $$ooai:zaguan.unizar.es:60668$$particulos$$pdriver
000060668 951__ $$a2019-07-09-11:34:26
000060668 980__ $$aARTICLE