60720 20190819101351.0 doi 10.1167/iovs.16-21100 sideral 98197 ART-2017-98197 eng (orcid)0000-0003-2770-4617 Ortillés, Á. Universidad de Zaragoza A rabbit model of Acanthamoeba keratitis: Use of infected soft contact lenses after corneal epithelium debridement with a diamond burr 2017 Access copy available to the general public Unrestricted PURPOSE. To develop a rabbit model of Acanthamoeba keratitis (AK) as the best method to reproduce the natural course of this disease. METHODS. To induce AK, infected contact lenses (1000 amoebae/mm2, 90% trophozoites) were placed over the previously debrided corneal surface, in combination with a temporary tarsorrhaphy. Environmental and clinical strains of Acanthamoeba spp. (genotype T4) were used. Three groups (1L, n = 32; 2L–21d, n = 5; 2L–3d, n = 23) were established according to the number of contact lenses used (1L, 1 lens; 2L–21d and 2L–3d, 2 lenses) and the placement day of these (1L, day 1; 2L–21d, days 1 and 21; 2L–3d, days 1 and 3). The infection was quantified by a clinical score system and confirmed using corneal cytology and culture, polymerase chain reaction and histopathologic analysis. RESULTS. The infection rate obtained was high (1L, 87.5%; 2L–21d, 100%; 2L–3d, 82.6%), although no clinical signs were observed in the 50% of the infected animals in group 1L. Among groups, group 2L–3d showed more cases of moderate and severe infection. Among strains, no statistically significant differences were found in the infection rate. In the control eyes, cross infection was confirmed when a sterile contact lens was placed in the previously debrided corneas but not if the eye remained intact. CONCLUSIONS. The combination of two infected contact lenses after corneal debridement seems to be an alternative model, clinically and histopathologically similar to its human counterpart, to induce the different AK stages and reproduce the course of the disease in rabbits. info:eu-repo/grantAgreement/ES/MINECO/DPI2014-54981-R info:eu-repo/grantAgreement/ES/MEC/FPU13-03782 info:eu-repo/grantAgreement/ES/DGA/T88 info:eu-repo/grantAgreement/ES/DGA/B124 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.388 2017 OPHTHALMOLOGY 9 / 59 = 0.153 2017 Q1 T1 2.058 2017 Cellular and Molecular Neuroscience 2017 Q1 Sensory Systems 2017 Q1 Ophthalmology 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0003-0765-7227 Goñi, P. Universidad de Zaragoza (orcid)0000-0002-9273-5885 Rubio, E. Universidad de Zaragoza (orcid)0000-0002-6134-8698 Sierra, M. Universidad de Zaragoza Gámez, E. (orcid)0000-0002-6455-119X Fernández, M.T. Universidad de Zaragoza Benito, M. Universidad de Zaragoza Cristóbal, J.Á. (orcid)0000-0001-9713-1813 Calvo, B. Universidad de Zaragoza 1008 660 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Parasitología 1006 255 Universidad de Zaragoza Dpto. Fisiatría y Enfermería Área Enfermería 1005 315 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Farmacología 1008 615 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Medic.Prevent.Salud Públ. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 58, 2 (2017), 1218-1227 Investig. ophthalmol. vis. sci. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 0146-0404 1342387 http://zaguan.unizar.es/record/60720/files/texto_completo.pdf Versión publicada 128074 http://zaguan.unizar.es/record/60720/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:60720 articulos driver 2019-08-19-09:51:17 ARTICLE