60883 20170503142843.0 doi 10.1128/JCM.02099-12 sideral 81810 ART-2013-81810 eng (orcid)0000-0001-5442-7702 Mainar-Jaime, R. Universidad de Zaragoza Sensitivity of the ISO 6579:2002/Amd 1:2007 standard method for detection of Salmonella spp. on mesenteric lymph nodes from slaughter pigs 2013 Access copy available to the general public Unrestricted The ISO 6579:2002/Amd 1:2007 (ISO) standard has been the bacteriological standard method used in the European Union for the detection of Salmonella spp. in pig mesenteric lymph nodes (MLN), but there are no published estimates of the diagnostic sensitivity (Se) of the method in this matrix. Here, the Se of the ISO (SeISO) was estimated on 675 samples selected from two populations with different Salmonella prevalences (14 farms with a =20% prevalence and 13 farms with a <20% prevalence) and through the use of latent-class models in concert with Bayesian inference, assuming 100% ISO specificity, and an invA-based PCR as the second diagnostic method. The SeISO was estimated to be close to 87%, while the sensitivity of the PCR reached up to 83.6% and its specificity was 97.4%. Interestingly, the bacteriological reanalysis of 33 potential false-negative (PCR-positive) samples allowed isolation of 19 (57.5%) new Salmonella strains, improving the overall diagnostic accuracy of the bacteriology. Considering the usual limitations of bacteriology regarding Se, these results support the adequacy of the ISO for the detection of Salmonella spp. from MLN and also that of the PCR-based method as an alternative or complementary (screening) test for the diagnosis of pig salmonellosis, particularly considering the cost and time benefits of the molecular procedure. info:eu-repo/grantAgreement/ES/INIA/FAU2008-16 info:eu-repo/grantAgreement/ES/INIA/RTA2007-65 info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 4.232 2013 MICROBIOLOGY 23 / 118 = 0.195 2013 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Andrés, S. (orcid)0000-0001-5313-7033 Vico, J.P. San Román, B. Garrido, V. Grilló, M.J. 1009 773 Universidad de Zaragoza Departamento de Patología Animal Sanidad Animal 51, 1 (2013), 89-94 J. clin. microbiol. JOURNAL OF CLINICAL MICROBIOLOGY 0095-1137 162123 http://zaguan.unizar.es/record/60883/files/texto_completo.pdf Versión publicada 132546 http://zaguan.unizar.es/record/60883/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:60883 articulos driver 2017-05-03-14:22:46 ARTICLE