000060904 001__ 60904
000060904 005__ 20190529115213.0
000060904 0247_ $$2doi$$a10.1073/pnas.1119912109
000060904 0248_ $$2sideral$$a76529
000060904 037__ $$aART-2012-76529
000060904 041__ $$aeng
000060904 100__ $$aFernandez-Fueyo, E.
000060904 245__ $$aComparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis
000060904 260__ $$c2012
000060904 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060904 5203_ $$aEfficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred to as white rot fungi. Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, Ceriporiopsis subvermispora, also depolymerizes lignin but may do so with relatively little cellulose degradation. To investigate the basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora and P. chrysosporium. Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium, respectively. In addition, the C. subvermispora genome contains at least seven genes predicted to encode laccases, whereas the P. chrysosporium genome contains none. We also observed expansion of the number of C. subvermispora desaturase-encoding genes putatively involved in lipid metabolism. Microarray-based transcriptome analysis showed substantial up-regulation of several desaturase and MnP genes in wood-containing medium. MS identified MnP proteins in C. subvermispora culture filtrates, but none in P. chrysosporium cultures. These results support the importance of MnP and a lignin degradation mechanism whereby cleavage of the dominant nonphenolic structures is mediated by lipid peroxidation products. Two C. subvermispora genes were predicted to encode peroxidases structurally similar to P. chrysosporium lignin peroxidase and, following heterologous expression in Escherichia coli, the enzymes were shown to oxidize high redox potential substrates, but not Mn2+. Apart from oxidative lignin degradation, we also examined cellulolytic and hemicellulolytic systems in both fungi. In summary, the C. subvermispora genetic inventory and expression patterns exhibit increased oxidoreductase potential and diminished cellulolytic capability relative to P. chrysosporium.
000060904 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/BIO2011-26694$$9info:eu-repo/grantAgreement/ES/MICINN/BIO2008-01533$$9info:eu-repo/grantAgreement/EC/FP7/265397/EU/Novel and more robust fungal peroxidases as industrial biocatalysts/PEROXICATS
000060904 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000060904 590__ $$a9.737$$b2012
000060904 591__ $$aMULTIDISCIPLINARY SCIENCES$$b4 / 57 = 0.07$$c2012$$dQ1$$eT1
000060904 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060904 700__ $$aRuiz-Dueñas, F.J.
000060904 700__ $$0(orcid)0000-0003-4076-6118$$aFerreira, P.$$uUniversidad de Zaragoza
000060904 700__ $$aFloudas, D.
000060904 700__ $$aHibbett, D.S.
000060904 700__ $$aCanessa, P.
000060904 700__ $$aLarrondo, L.F.
000060904 700__ $$aJames, T.Y.
000060904 700__ $$aSeelenfreund, D.
000060904 700__ $$aLobos, S.
000060904 700__ $$aPolanco, R.
000060904 700__ $$aTello, M.
000060904 700__ $$aHonda, Y.
000060904 700__ $$aWatanabe, T.
000060904 700__ $$aWatanabe, T.
000060904 700__ $$aSan, R.J.
000060904 700__ $$aKubicek, C.P.
000060904 700__ $$aSchmoll, M.
000060904 700__ $$aGaskell, J.
000060904 700__ $$aHammel, K.E.
000060904 700__ $$aSt John, F.J.
000060904 700__ $$aWymelenberg, A.V.
000060904 700__ $$aSabat, G.
000060904 700__ $$aBonDurant, S.S.
000060904 700__ $$aSyed, K.
000060904 700__ $$aYadav, J.S.
000060904 700__ $$aDoddapaneni, H.
000060904 700__ $$aSubramanian, V.
000060904 700__ $$aLaviñ, J.L.
000060904 700__ $$aOguiza, J.A.
000060904 700__ $$aPerez, G.
000060904 700__ $$aPisabarro, A.G.
000060904 700__ $$aRamirez, L.
000060904 700__ $$aSantoyo, F.
000060904 700__ $$aMaster, E.
000060904 700__ $$aCoutinho, P.M.
000060904 700__ $$aHenrissat, B.
000060904 700__ $$aLombard, V.
000060904 700__ $$aMagnuson, J.K.
000060904 700__ $$aKuës, U.
000060904 700__ $$aHori, C.
000060904 700__ $$aIgarashi, K.
000060904 700__ $$aSamejima, M.
000060904 700__ $$aHeld, B.W.
000060904 700__ $$aBarry, K.W.
000060904 700__ $$aLaButti, K.M.
000060904 700__ $$aLapidus, A.
000060904 700__ $$aLindquist, E.A.
000060904 700__ $$aLucas, S.M.
000060904 700__ $$aRiley, R.
000060904 700__ $$aSalamov, A.A.
000060904 700__ $$aHoffmeister, D.
000060904 700__ $$aSchwenk, D.
000060904 700__ $$aHadar, Y.
000060904 700__ $$aYarden, O.
000060904 700__ $$aDe Vries, R.P.
000060904 700__ $$aWiebenga, A.
000060904 700__ $$aStenlid, J.
000060904 700__ $$aEastwood, D.
000060904 700__ $$aGrigoriev, I.V.
000060904 700__ $$aBerka, R.M.
000060904 700__ $$aBlanchette, R.A.
000060904 700__ $$aKersten, P.
000060904 700__ $$aMartinez, A.T.
000060904 700__ $$aVicuna, R.
000060904 700__ $$aCullen, D.
000060904 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000060904 773__ $$g109, 14 (2012)$$pProc. Natl. Acad. Sci.$$tProceedings of the National Academy of Sciences$$x0027-8424
000060904 8564_ $$s8533242$$uhttps://zaguan.unizar.es/record/60904/files/texto_completo.pdf$$yVersión publicada
000060904 8564_ $$s158000$$uhttps://zaguan.unizar.es/record/60904/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
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000060904 951__ $$a2019-05-29-11:40:15
000060904 980__ $$aARTICLE