60968 20240118091946.0 doi 10.3390/ijms18030662 sideral 98505 ART-2017-98505 eng González-Arto, M. (orcid)0000-0003-2745-4152 Melatonin MT1 and MT2 receptors in the ram reproductive tract 2017 Access copy available to the general public Unrestricted Some melatonin functions in mammals are exerted through MT1 and MT2 receptors. However, there are no reports of their presence in the reproductive tract of the ram, a seasonal species. Thus, we have investigated their existence in the ram testis, epididymis, accessory glands and ductus deferens. Real-time polymerase chain reaction (qPCR) revealed higher levels of m-RNA for both receptors in the testis, ampulla, seminal vesicles, and vas deferens, than in the other organs of the reproductive tract (p < 0.05). Western blot analyses showed protein bands compatible with the MT1 in the testis and cauda epididymis, and for the MT2 in the cauda epididymis and deferent duct. Immunohistochemistry analyses revealed the presence of MT1 receptors in spermatogonias, spermatocytes, and spermatids, and MT2 receptors in the newly-formed spermatozoa in the testis, whereas both receptors were located in the epithelial cells of the ampulla, seminal vesicles, and ductus deferens. Indirect immunofluorescence showed significant differences in the immunolocation of both receptors in spermatozoa during their transit in the epididymis. In conclusion, it was demonstrated that melatonin receptors are present in the ram reproductive tract. These results open the way for new studies on the molecular mechanism of melatonin and the biological significance of its receptors. info:eu-repo/grantAgreement/ES/DGA/A26 info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.687 2017 CHEMISTRY, MULTIDISCIPLINARY 52 / 169 = 0.308 2017 Q2 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 90 / 292 = 0.308 2017 Q2 T1 1.26 2017 Medicine (miscellaneous) 2017 Q1 Physical and Theoretical Chemistry 2017 Q1 Computer Science Applications 2017 Q1 Inorganic Chemistry 2017 Q1 Spectroscopy 2017 Q1 Organic Chemistry 2017 Q1 Molecular Biology 2017 Q2 Catalysis 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Aguilar, D. (orcid)0000-0001-5940-3339 Gaspar-Torrubia, E. Gallego, M. Universidad de Zaragoza (orcid)0000-0003-3945-4854 Carvajal-Serna, M. (orcid)0000-0003-3929-5064 Herrera-Marcos, L. Universidad de Zaragoza (orcid)0000-0002-4665-9674 Serrano-Blesa, E. (orcid)0000-0001-5276-7834 Dos, Santos Hamilton Pérez-Pé, R. Universidad de Zaragoza (orcid)0000-0002-2312-6402 Muiño-Blanco, T. Universidad de Zaragoza (orcid)0000-0003-1306-8517 Cebrián-Pérez, J. A. Universidad de Zaragoza (orcid)0000-0001-9842-6197 Casao, A. Universidad de Zaragoza (orcid)0000-0003-1997-4262 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 18, 3 (2017), 662 [15 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 8405029 http://zaguan.unizar.es/record/60968/files/texto_completo.pdf Versión publicada 100913 http://zaguan.unizar.es/record/60968/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:60968 articulos driver 2024-01-18-09:10:25 ARTICLE