61356 20210121114510.0 doi 10.1016/j.ymeth.2014.09.010 sideral 89846 ART-2015-89846 eng (orcid)0000-0002-1232-6310 Vega, S. A unified framework based on the binding polynomial for characterizing biological systems by isothermal titration calorimetry 2015 Access copy available to the general public Unrestricted Isothermal titration calorimetry (ITC) has become the gold-standard technique for studying binding processes due to its high precision and sensitivity, as well as its capability for the simultaneous determination of the association equilibrium constant, the binding enthalpy and the binding stoichiometry. The current widespread use of ITC for biological systems has been facilitated by technical advances and the availability of commercial calorimeters. However, the complexity of data analysis for non-standard models is one of the most significant drawbacks in ITC. Many models for studying macromolecular interactions can be found in the literature, but it looks like each biological system requires specific modeling and data analysis approaches. The aim of this article is to solve this lack of unity and provide a unified methodological framework for studying binding interactions by ITC that can be applied to any experimental system. The apparent complexity of this methodology, based on the binding polynomial, is overcome by its easy generalization to complex systems. info:eu-repo/grantAgreement/ES/DGA/B01 info:eu-repo/grantAgreement/ES/DGA/B89 info:eu-repo/grantAgreement/ES/FIS/PI10-00186 info:eu-repo/grantAgreement/ES/ISCIII/CP07-00289 info:eu-repo/grantAgreement/ES/MICINN/BFU2010-19451 info:eu-repo/grantAgreement/ES/MICINN/PTA2009-2341-I info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.503 2015 BIOCHEMICAL RESEARCH METHODS 18 / 77 = 0.234 2015 Q1 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 99 / 289 = 0.343 2015 Q2 T2 2.367 2015 Molecular Biology 2015 Q1 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2015 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0001-5664-1729 Abian, O. (orcid)0000-0001-5702-4538 Velazquez-Campoy, A. Universidad de Zaragoza 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 76 (2015), 99-115 Methods METHODS 1046-2023 620182 http://zaguan.unizar.es/record/61356/files/texto_completo.pdf Postprint 41555 http://zaguan.unizar.es/record/61356/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:61356 articulos driver 2021-01-21-10:56:15 ARTICLE