000061468 001__ 61468
000061468 005__ 20190709135450.0
000061468 0247_ $$2doi$$a10.1038/s41598-017-00583-0
000061468 0248_ $$2sideral$$a98848
000061468 037__ $$aART-2017-98848
000061468 041__ $$aeng
000061468 100__ $$0(orcid)0000-0001-6479-3933$$aOlaciregui, Maite$$uUniversidad de Zaragoza
000061468 245__ $$aIn vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
000061468 260__ $$c2017
000061468 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061468 5203_ $$aFreeze-drying (FD) is a new and alternative method to preserve spermatozoa in refrigeration or at room temperature. Suitable protection is required to maintain the sperm DNA integrity during the whole process and storage. The aim of this study was to examine the effect of rosmarinic acid and storage temperature on the DNA integrity of freeze-dried ram sperm. In addition, we evaluated the in vitro developmental ability to the blastocyst stage of oocytes injected with freeze-dried sperm. Ram sperm was freeze-dried in basic medium and in this medium supplemented with 105¿µM rosmarinic acid. The vials were stored for 1 year at 4¿°C and at room temperature. Frozen sperm was used as control. After rehydration, sperm DNA damage was evaluated, observing that the percentage of spermatozoa with DNA damage decreased significantly in the presence of rosmarinic acid, without differences between the two storage temperatures. Moreover, no differences were observed between the freeze-dried group and the frozen-thawed group in terms of blastocyst formation rate. We proved for the first time that ovine spermatozoa can be lyophilized effectively, stored at room temperature for long term, reconstituted and further injected into oocytes with initial embryo development.
000061468 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061468 590__ $$a4.122$$b2017
000061468 591__ $$aMULTIDISCIPLINARY SCIENCES$$b12 / 64 = 0.188$$c2017$$dQ1$$eT1
000061468 592__ $$a1.533$$b2017
000061468 593__ $$aMultidisciplinary$$c2017$$dQ1
000061468 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061468 700__ $$0(orcid)0000-0002-7857-9732$$aLuño, Victoria$$uUniversidad de Zaragoza
000061468 700__ $$0(orcid)0000-0002-1611-6337$$aGonzález, Noelia$$uUniversidad de Zaragoza
000061468 700__ $$0(orcid)0000-0002-0797-1841$$aGil, Lydia$$uUniversidad de Zaragoza
000061468 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000061468 773__ $$g7 (2017), 1096 [8 pp.]$$pSci. rep.$$tScientific reports$$x2045-2322
000061468 8564_ $$s1423129$$uhttps://zaguan.unizar.es/record/61468/files/texto_completo.pdf$$yVersión publicada
000061468 8564_ $$s110104$$uhttps://zaguan.unizar.es/record/61468/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061468 909CO $$ooai:zaguan.unizar.es:61468$$particulos$$pdriver
000061468 951__ $$a2019-07-09-11:40:14
000061468 980__ $$aARTICLE