61469 20200428120340.0 doi 10.9755/ejfa.2016-11-1585 sideral 98854 ART-2017-98854 eng Fatnassi, Meriem Identification of ß-nerve growth factor in dromedary camel seminal plasma and its role in induction of ovulation in females 2017 Access copy available to the general public Unrestricted The main objective of this study was to demonstrate the effect of seminal plasma ovulation inducing factor (OIF) on ovulation in female camels. Seminal plasma was fractionated using gel filtration chromatography, and two protein peaks were obtained and analysed by western blotting. The effect of the bioactive protein fraction (OIF/ß-NGF) was tested by intramuscular injection (1 ml) in synchronized females with the following treatments: PBS (negative control group, n=3); 20 /jg Buserelin (GnRH analogue, positive control group; n=3); and purified OIF with doses of 1 and 2 mg (n = 5, each treatment). Blood samples were collected every two days from day 0 until 14 days post-treatment, and the progesterone concentration was assessed. The obtained results showed that the OIF is highly present in the seminal plasma of dromedary camels as a protein with a molecular mass of approximately 14 kDa. It was detected as a betaNerve Growth Factor, named Cam-ß-NGF. The effect of this molecule on ovulation was clearly demonstrated by the significant increase of the plasma progesterone concentration in the treated female groups (1 and 2 mg of Cam-ß-NGF) as observed in Buserelin group. In summary, intramuscular injection of ß-NGF isolated from dromedary camel seminal plasma induces ovulation in females with similar rate to Buserelin treatment. info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P info:eu-repo/grantAgreement/ES/DGA/A26 info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 0.609 2017 AGRONOMY 66 / 87 = 0.759 2017 Q4 T3 FOOD SCIENCE & TECHNOLOGY 110 / 133 = 0.827 2017 Q4 T3 0.27 2017 Agronomy and Crop Science 2017 Q3 Food Science 2017 Q3 Applied Microbiology and Biotechnology 2017 Q3 Animal Science and Zoology 2017 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0001-9842-6197 Cebrián-Pérez, José Alvaro Universidad de Zaragoza Salhi, Imed (orcid)0000-0002-2312-6402 Pérez-Pé, Rosaura Universidad de Zaragoza Seddik, Mabrouk Mouldi (orcid)0000-0003-1997-4262 Casao, Adriana Universidad de Zaragoza Khorchani, Touhami (orcid)0000-0003-1306-8517 Muiño-Blanco, Teresa Universidad de Zaragoza Hammadi, Mohamed 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 29, 4 (2017), 293-299 Emir. j. food agric. Emirates Journal of Food and Agriculture 2079-052X 739188 http://zaguan.unizar.es/record/61469/files/texto_completo.pdf Versión publicada 111924 http://zaguan.unizar.es/record/61469/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:61469 articulos driver 2020-04-28-12:01:09 ARTICLE