61493 20190529115218.0 doi 10.1371/journal.pone.0065243 sideral 83186 ART-2013-83186 eng Cabezas-Cruz, A. Functional and Immunological Relevance of Anaplasma marginale Major Surface Protein 1a Sequence and Structural Analysis 2013 Access copy available to the general public Unrestricted Bovine anaplasmosis is caused by cattle infection with the tick-borne bacterium, Anaplasma marginale. The major surface protein 1a (MSP1a) has been used as a genetic marker for identifying A. marginale strains based on N-terminal tandem repeats and a 5'-UTR microsatellite located in the msp1a gene. The MSP1a tandem repeats contain immune relevant elements and functional domains that bind to bovine erythrocytes and tick cells, thus providing information about the evolution of host-pathogen and vector-pathogen interactions. Here we propose one nomenclature for A. marginale strain classification based on MSP1a. All tandem repeats among A. marginale strains were classified and the amino acid variability/frequency in each position was determined. The sequence variation at immunodominant B cell epitopes was determined and the secondary (2D) structure of the tandem repeats was modeled. A total of 224 different strains of A. marginale were classified, showing 11 genotypes based on the 5'-UTR microsatellite and 193 different tandem repeats with high amino acid variability per position. Our results showed phylogenetic correlation between MSP1a sequence, secondary structure, B-cell epitope composition and tick transmissibility of A. marginale strains. The analysis of MSP1a sequences provides relevant information about the biology of A. marginale to design vaccines with a cross-protective capacity based on MSP1a B-cell epitopes. info:eu-repo/grantAgreement/ES/MINECO/BFU2011-23896 info:eu-repo/grantAgreement/EC/FP7/238511/EU/Post-graduate training network for capacity building to control ticks and tick-borne diseases/POSTICK info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.534 2013 MULTIDISCIPLINARY SCIENCES 8 / 56 = 0.143 2013 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Passos, L.M.F. Lis, K. Kenneil, R. Valdés, J.J. Ferrolho, J. Tonk, M. Pohl, A.E. Grubhoffer, L. Zweygarth, E. Shkap, V. Ribeiro, M.F.B. (orcid)0000-0001-7483-046X Estrada-Peña, A. Universidad de Zaragoza Kocan, K.M. de la Fuente, J. 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 8, 6 (2013), e65243 [13 pp] PLoS One PLoS ONE 1932-6203 4270454 http://zaguan.unizar.es/record/61493/files/texto_completo.pdf Versión publicada 125430 http://zaguan.unizar.es/record/61493/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:61493 articulos driver 2019-05-29-11:40:58 ARTICLE