000061497 001__ 61497
000061497 005__ 20170612112602.0
000061497 0247_ $$2doi$$a10.1038/ncomms3546
000061497 0248_ $$2sideral$$a83243
000061497 037__ $$aART-2013-83243
000061497 041__ $$aeng
000061497 100__ $$aPadavannil, A.
000061497 245__ $$aStructure of GrlR-GrlA complex that prevents GrlA activation of virulence genes
000061497 260__ $$c2013
000061497 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061497 5203_ $$aThe locus of enterocyte effacement (LEE) is essential for virulence of enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). The 41 genes of the LEE encode type III secretion system proteins and three associated regulators: Ler, GrlA and GrlR. Ler is a positive regulator for most of the LEE operons, including grlRA. GrlA controls the expression of ler, ehxCABD and flhDC operons. GrlR binds to GrlA and suppresses its function. Here we report the crystal structure of GrlR–GrlA¿ (aa 1–106) complex (2:1) and its functional characterization. We show that GrlR interacts with the Helix-Turn-Helix motif of GrlA. Moreover, GrlA binds to the promoter DNA fragments of ler, ehxCABD and flhDC, and GrlR outcompetes with these promoter DNA sequences for the Helix-Turn-Helix motif of GrlA. These findings provide mechanistic insight into a regulatory module for the virulence of EPEC and EHEC, two important pathogens that cause devastating diseases.
000061497 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061497 590__ $$a10.742$$b2013
000061497 591__ $$aMULTIDISCIPLINARY SCIENCES$$b3 / 55 = 0.055$$c2013$$dQ1$$eT1
000061497 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061497 700__ $$aJobichen, C.
000061497 700__ $$aMills, E.
000061497 700__ $$0(orcid)0000-0001-5702-4538$$aVelazquez-Campoy, A.$$uUniversidad de Zaragoza
000061497 700__ $$aLi, M.
000061497 700__ $$aLeung, K.Y.
000061497 700__ $$aMok, Y.K.
000061497 700__ $$aRosenshine, I.
000061497 700__ $$aSivaraman, J.
000061497 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDepartamento de Bioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular
000061497 773__ $$g4 (2013), 2546 [10 pp]$$pNATURE COMMUNICATIONS$$tNATURE COMMUNICATIONS$$x2041-1723
000061497 8564_ $$s1205621$$uhttps://zaguan.unizar.es/record/61497/files/texto_completo.pdf$$yVersión publicada
000061497 8564_ $$s62798$$uhttps://zaguan.unizar.es/record/61497/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061497 909CO $$ooai:zaguan.unizar.es:61497$$particulos$$pdriver
000061497 951__ $$a2017-06-12-09:40:20
000061497 980__ $$aARTICLE