61501 20170612112603.0 doi 10.4081/ijas.2013.e66 sideral 98755 ART-2013-98755 eng Ceccobelli, S. Phylogeny, genetic relationships and population structure of five Italian local chicken breeds 2013 Access copy available to the general public Unrestricted Number and population size of local chicken breeds in Italy is considered to be critical. Molecular data can be used to provide reliable insight into the diversity of chicken breeds. The first aim of this study was to investigate the maternal genetic origin of five Italian local chicken breeds (Ancona, Livorno, Modenese, Romagnola and Valdarnese bianca) based on mitochondrial DNA (mtDNA) information. Secondly, the extent of the genetic diversity, population structure and the genetic relationships among these chicken populations, by using 27 microsatellite markers, were assessed. To achieve these targets, a 506 bp fragment of the D-loop region was sequenced in 50 chickens of the five breeds. Eighteen variable sites were observed which defined 12 haplotypes. They were assigned to three clades and two maternal lineages. Results indicated that 90% of the haplotypes are related to clade E, which has been described to originate from the Indian subcontinent. For the microsatellite analysis, 137 individual blood samples from the five Italian breeds were included. A total of 147 alleles were detected at 27 microsatellite loci. The five Italian breeds showed a slightly higher degree of inbreeding (FIS=0.08) than the commercial populations that served as reference. Structure analysis showed a separation of the Italian breeds from the reference populations. A further sub-clustering allowed discriminating among the five different Italian breeds. This research provides insight into population structure, relatedness and variability of the five studied breeds. info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 0.604 2013 VETERINARY SCIENCES 92 / 131 = 0.702 2013 Q3 T3 AGRICULTURE, DAIRY & ANIMAL SCIENCE 30 / 52 = 0.577 2013 Q3 T2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Di Lorenzo, P. Lancioni, H. Castellini, C. (orcid)0000-0003-0210-2919 Monteagudo Ibáñez, L.V. Universidad de Zaragoza Sabbioni, A. Sarti, F.M. Weigend, S. Lasagna, E. 1001 420 Universidad de Zaragoza Departamento de Anatomía, Embriología y Genética Animal Genética 12, 3 (2013), 410-417 Ital. J. Anim. Sci. Italian Journal of Animal Science 1594-4077 503785 http://zaguan.unizar.es/record/61501/files/texto_completo.pdf Versión publicada 20093 http://zaguan.unizar.es/record/61501/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:61501 articulos driver 2017-06-12-09:40:38 ARTICLE