61593 20240122154813.0 doi 10.1177/2050640616663689 sideral 99463 ART-2016-99463 eng Layunta, E. Universidad de Zaragoza (orcid)0000-0001-5573-6144 Toll-like receptor 9 modifies intestinal serotonergic system. 2016 Access copy available to the general public Unrestricted Introduction: Toll-like receptor 9 (TLR9) is expressed in intestinal epithelial cells, which recognize microbiota developing different responses 1. Several studies have shown that TLR9 seems to be involved in Inflammatory Bowel Diseases (IBD) due to an inappropriate defensive response against microorganisms 2. Moreover, intestinal serotonergic system is also altered in IBD, where extracellular serotonin (5–HT) levels are increased 3. 5-HT bioavailability is mainly regulated by the serotonin transporter (SERT), expressed in enterocytes 4. Aims & Methods: The aim of the present study was to analyse whether TLR9 activation affects SERT expression and activity, and expression of other elements from the serotonergic system (TPH1, TPH2 and 5-HT receptors). Human enterocyte-like Caco-2 cells, and ileum and colon from TLR9-/- mice and Dextran Sulphate Sodium (DSS) mouse colitis model were used as experimental models. mRNA expression was determined by RT-qPCR, and protein expression by western blot... info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 3.673 2016 GASTROENTEROLOGY & HEPATOLOGY 21 / 79 = 0.266 2016 Q2 T1 1.137 2016 Oncology 2016 Q2 Gastroenterology 2016 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Latorre, E. Forcén, R. Grasa, L. Universidad de Zaragoza (orcid)0000-0002-5306-9365 Pardo, J. Universidad de Zaragoza (orcid)0000-0003-0154-0730 Castro, M. Plaza, M.A. Universidad de Zaragoza (orcid)0000-0002-7412-2073 Arruebo, M.P. Universidad de Zaragoza (orcid)0000-0002-2114-0577 Mesonero, J.E. Universidad de Zaragoza (orcid)0000-0003-4758-3998 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 4, Suppl. 1 (2016), A426-A426 United European Gastroenterol. j. United European Gastroenterology Journal 2050-6406 96221 http://zaguan.unizar.es/record/61593/files/texto_completo.pdf Versión publicada 123061 http://zaguan.unizar.es/record/61593/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:61593 articulos driver 2024-01-22-15:31:06 ARTICLE