Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines /631/1647/767/70 /631/154/1435 /123 /13/21 /14/63 /13/31 article
Resumen: We describe a novel protocol to quantitatively and simultaneously compare the chemotactic responses of cells towards different chemokines. In this protocol, droplets of agarose gel containing different chemokines are applied onto the surface of a Petri dish, and then immersed under culture medium in which cells are suspended. As chemokine molecules diffuse away from the spot, a transient chemoattractant gradient is established across the spots. Cells expressing the corresponding cognate chemokine receptors migrate against this gradient by crawling under the agarose spots towards their centre. We show that this migration is chemokine-specific; meaning that only cells that express the cognate chemokine cell surface receptor, migrate under the spot containing its corresponding chemokine ligand. Furthermore, we show that migration under the agarose spot can be modulated by selective small molecule antagonists present in the cell culture medium.
Idioma: Inglés
DOI: 10.1038/s41598-017-00949-4
Año: 2017
Publicado en: Scientific reports 7, 1 (2017), 1075 [11 pp]
ISSN: 2045-2322

Factor impacto JCR: 4.122 (2017)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 12 / 64 = 0.188 (2017) - Q1 - T1
Factor impacto SCIMAGO: 1.533 - Multidisciplinary (Q1)

Tipo y forma: Article (Published version)

Creative Commons You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.


Exportado de SIDERAL (2019-07-09-11:38:05)

Este artículo se encuentra en las siguientes colecciones:
Articles



 Record created 2017-06-29, last modified 2019-07-09


Versión publicada:
 PDF
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)