61676 20230421130005.0 doi 10.1016/j.vaccine.2013.07.051 sideral 85742 ART-2013-85742 eng Arbues, Ainhoa Construction, characterization and preclinical evaluation of MTBVAC, the first live-attenuated M. tuberculosis-based vaccine to enter clinical trials 2013 Access copy available to the general public Unrestricted The development of a new tuberculosis vaccine is an urgent need due to the failure of the current vaccine, BCG, to protect against the respiratory form of the disease. MTBVAC is an attenuated Mycobacterium tuberculosis vaccine candidate genetically engineered to fulfil the Geneva consensus requirements to enter human clinical trials. We selected a M. tuberculosis clinical isolate to generate two independent deletions without antibiotic-resistance markers in the genes phoP, coding for a transcription factor key for the regulation of M. tuberculosis virulence, and fadD26, essential for the synthesis of the complex lipids phthiocerol dimycocerosates (DIM), one ofthe major mycobacterial virulence factors. The resultant strain MTBVAC exhibits safety and biodistribution profiles similar to BCG and confers superior protection in preclinical studies. These features have enabled MTBVAC to be the first live attenuated M. tuberculosis vaccine to enter clinical evaluation. info:eu-repo/grantAgreement/EC/FP7/241745/EU/Discovery and preclinical development of new generation tuberculosis vaccines/NEWTBVAC info:eu-repo/grantAgreement/ES/MICINN/Juan de la Cierva Program-JCI-2009-03799 info:eu-repo/grantAgreement/ES/MINECO/BES-2006-11950 info:eu-repo/grantAgreement/ES/MINECO/BIO2008-01561 info:eu-repo/grantAgreement/ES/MINECO/BIO2011-23555 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.485 2013 MEDICINE, RESEARCH & EXPERIMENTAL 35 / 124 = 0.282 2013 Q2 T1 IMMUNOLOGY 53 / 144 = 0.368 2013 Q2 T2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Aguilo, Juan I. Universidad de Zaragoza (orcid)0000-0001-7897-9173 Gonzalo-Asensio, Jesús Universidad de Zaragoza (orcid)0000-0001-8841-6593 Marinova, Dessislava Uranga, Santiago Puentes, Eugenia Fernández, Conchita Parra, Alberto Cardona, Pere Joan Vilaplana, Cristina Ausina, Vicente Williams, Ann Clark, Simon Malaga, Wladimir Guilhot, Christophe Gicquel, Brigitte Martin, Carlos Universidad de Zaragoza (orcid)0000-0003-2993-5478 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 31, 42 (2013), 4867-4873 Vaccine VACCINE 0264-410X 1841361 http://zaguan.unizar.es/record/61676/files/texto_completo.pdf Postprint 91032 http://zaguan.unizar.es/record/61676/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:61676 articulos driver 2023-04-21-12:39:25 ARTICLE