Nuclear localization of the CK2a-subunit correlates with poor prognosis in clear cell renal cell carcinoma

Rabjerg, M. ; Guerra, B. ; Oliván-Viguera, A. ; Mikkelsen, M.L.N. ; Köhler, R. ; Issinger, O.G. ; Marcussen, N.

Resumen: Protein kinase CK2a, one of the two catalytic isoforms of the protein kinase CK2 has been shown to contribute to tumor development, tumor proliferation and suppression of apoptosis in various malignancies. We conducted this study to investigate CK2 expression in different subtypes of Renal Cell Carcinoma (RCC) and in the benign oncocytoma. qRT-PCR, immunohistochemistry and Western blot analyses revealed that CK2a expression was significantly increased at the mRNA and protein levels in clear cell RCC (ccRCC). Also the kinase activity of CK2 was significantly increased in ccRCC compared to normal renal cortex. Nuclear protein expression of CK2a correlated in univariate analysis with poor Progression Free Survival (HR = 8.11, p = 0.016). Functional analyses (cell proliferation assay) revealed an inhibitory effect of Caki-2 cell growth following CK2 inhibition with CX-4945. Our results suggest that CK2a promotes migration and invasion of ccRCC and therefore could serve as a novel prognostic biomarker and molecular therapeutic target in this type of cancer.

Idioma: Inglés

DOI: 10.18632/oncotarget.13693

Año: 2017

Publicado en: Oncotarget 8, 1 (2017), 1613-1627
ISSN: 1949-2553

Factor impacto SCIMAGO: 1.942 - Oncology (Q1)

Tipo y forma: Artículo (Versión definitiva)